Abstract
Several media are proposed particularly for the detection of Aeromonas spp. but also for Plesiomonas shigelloides and Pseudomonas spp. Some are for general purposes and others specifically for the examination of clinical, environmental or food samples. All media are selective, due to antibiotics, bile salts, dyes and other selective agents, as well as differential, primarily based on the ability of the microorganisms to ferment/not ferment carbohydrates. As with all selective media, the recovery of stressed cells is sometimes prevented and the competing flora is not always completely inhibited so that confirmatory tests need to be made on presumptive positive colonies. The choice of a specific medium for isolation of Aeromonas spp. will always depend on the type of sample to be examined and whether the investigator needs qualitative detection or quantitative recovery. The best medium for quantitative estimation of Aeromonas spp. from food and environmental samples seems to be starch ampicillin agar (SAA), though others might be recommended. There is a need for a comparative study including Rippey Cabelli agar (mA), ampicillin bile salts inositol xylose (MIX) agar, ampicillin dextrin agar (ADA), dextrin fuchsin sulphite agar (DFS) and starch glutamate ampicillin penicillin C-glucose agar (SGAP-10C) in addition to SAA. For routine analysis of environmental and food samples for P. shigelloides , spread plating on inositol brilliant green bile salts (IBB) and plesiomonas (PL) agars is recommended. For Pseudomonas spp., CFC agar permits quantitative recovery of both pigmented and non-pigmented strains from food and environmental samples, whilst at the same time inhibiting most other organisms.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.