Abstract
The research and industrialization of genetically modified organisms (GMO) has gained much attention in the past few decades. Biotechnology gradually became a powerful tool for future development and a new level of economy and science. Detection methods based on the polymerase chain reaction (PCR) are the principal approach for detection of these products. In this chapter, we systematically describe validation of DNA-based detection methods, based on the experience of GMO testing laboratories. We cover standards and guidelines, method validation, data interpretation, reference material, and proficiency testing. The main issues of the above five areas are discussed, to serve as a reference for those carrying out GMO analysis.
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