Abstract
Publisher Summary Immunofluorescence procedures to analyze the in situ distribution patterns of specific chromosomal proteins on Drosophila polytene chromosomes have been developed in past years. This chapter focuses on a set of results that have been obtained with this technique indicating the distribution patterns of nonhistone chromosomal (NHC) proteins, which may reflect structural differences in chromatin related to the control of gene expression. The technique of in situ hybridization is used to localize the complements of particular DNA and RNA sequences to particular polytene chromosome bands. By using antisera against specific chromosomal proteins, the pattern of distribution of these components in relation to the established banding pattern can be similarly assessed. It is of particular interest to consider the distribution pattern of chromosomal proteins in relation to the patterns of gene activity. The evidence for at least three types of NHC proteins bearing different relationships to active and inactive genes is relatively convincing—namely, (1) those widely distributed in chromatin, (2) those associated with the developmentally active loci and heat shock-activated loci of the third instar salivary gland, and (3) those associated primarily with active loci such as RNA polymerase II.
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