Abstract
In living systems, proteins are organized and oriented to deliver signals with precision and specificity. Protein components are widely incorporated into tissue engineering constructs. This chapter discusses how we can integrate signaling proteins into tissue engineering to optimize bioreaction specificity and signal-to-noise. The two major sections of this review article address (1) techniques to measure protein orientation, and (2) methods for orienting proteins to deliver cell communication signals better. Analytical methods briefly described here are X-ray photoelectron spectroscopy, secondary ion mass spectrometry, sum frequency generation, surface plasmon resonance, quartz crystal microbalance with dissipation monitoring, and atomic force microscopy. Methods for orienting, controlling, and stabilizing proteins described in this chapter include histidine residue tags, collagen type I, lipid bilayer methods, streptavidin, and hydroxyapatite.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
