Abstract
Publisher Summary Liquid column chromatography and thin-layer chromatography are the most important techniques for the purification and fractionation of lipids, the former being mainly used for preparative and the latter for analytical purposes. The sample often has to be purified prior to the fractionation by removing water and nonlipidic contaminants—e.g., by chromatography on a Sephadex G-25 column especially if it has been obtained by extraction with chloroform and methanol. Lipid–protein complexes may be present that can be resolved by chromatography on cross-linked polystyrene gels. Prostaglandins are best isolated from natural biological material by the extraction and purification of the extract by silica gel column chromatography combined with preparative thin-layer chromatography. The phospholipidic and glycolipidic fractions, isolated by the methods of separation into lipid classes, can be further sub-fractionated by various column chromatographic procedures. For the major components, combinations of column and thin-layer chromatography can be used, while for the minor components, the use of preparative column chromatography is necessary, even for the sub-fractionation.
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