Abstract
Publisher Summary This chapter focuses on the use of root border cells as tools in plant cell studies. Root border cells lend themselves to a wide range of studies by virtue of their unique morphology. Border cells are populations of single cells that separate from the outer cells of the root cap and can be collected by gentle agitation in water. Substantial evidence is consistent with the hypothesis that border cells constitute a uniquely differentiated part of the root system that functions to modulate microbial populations in the rhizosphere. However, as populations of single, metabolically active cells with intact cell walls, root border cells provide tools to study a wide range of biological questions. Unlike protoplasts, border cells survive in a wide range of osmotic conditions, including distilled water, without lysis or observable cellular damage. The chapter provides specific conditions for the manipulation of pea root border cells. Diverse applications of root border cells in assays for research and teaching are also discussed.
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