Abstract
This chapter focuses on the regulation of muscarinic acetylcholine receptors (mAChR) expression and function when expressed from endogenous genes in cardiac cells and when expressed from heterologous genes in transfected cells in culture. Muscarinic receptors play a key role in the functioning of the central nervous system, where they have been implicated in such processes as memory, learning, and control of movement. Muscarinic receptors also modulate synaptic transmission in the ganglia of the autonomic nervous system and play a major role in regulating the functions of the target organs of the parasympathetic nervous system. The chapter describes several techniques of molecular and cellular biology to identify the factors that regulate the expression and function of mAChR in order to understand how cells regulate their ability to participate in cholinergic synaptic transmission. The regulation of expression of a luciferase reporter gene under the transcriptional control of a CAMP-regulated promoter is used to study the functional specificity of mAChR in transiently transfected cells.
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