Changes of plasma osmolality, chloride concentration and gill Na?K-ATPase activity in tilapia Oreochromis mossambicus during seawater acclimation

Abstract

Changes of plasma osmolality, chloride concentration and gill Na−K-ATPase activity in tilapia Oreochromis mossambicus (obtained from Tainan Fish Culture Station of Taiwan Fisheries Research Institute, 1987) during seawater acclimation were examined. Three experiments were performed. (1) Freshwater (FW) to 30‰ salinity seawater (SW): plasma osmolality and chloride rose violently immediately post-transfer. At 6 h, gill Na−K-ATPase activity began to increase but most fish died from excessive plasma osmolality and Cl. (2) FW to 20‰ salinity SW: plasma osmolality and chloride increased immediately post-transfer, but more slowly than in (1), and began to decrease at 24 h. However it was not until 12 h post-transfer that gill Na−K-ATPase activity rose slowly. (3) FW to 20‰ salinity SW for 24 h, then to 30‰ salinity SW: after transfer to 30‰ salinity, plasma osmolality and chloride showed only a small increase initially then declined, while gill Na−K-ATPase activity started to rise rapidly within 3 h. The present results coincided with our previous morphological data concerning the ultrastructural responses of gill chloride cells. These are discussed to elucidate the osmoregulation mechanisms in tilapia during seawater acclimation.