Abstract

Objective To study changes of plasma microRNA (miRNA) of rats in myocardial injury after cardiopulmonary resuscitation and their significance. Methods A total of 64 Sprague-Dawley rats were divided into the control group and the experimental group, 32 rats in each group. The rat model of cardiopulmonary resuscitation was established by asphyxia. The levels of plasma miRNA-1, miRNA-21, miRNA-126, miRNA-133a, miRNA-210, miRNA-320 and miRNA-499 were detected at different time points when the two groups received venous blood after tracheotomy (the control group) or cardiopulmonary resuscitation (the experimental group) 3, 6, 12, 24 h respectively. The area under the receiver operating characteristic (ROC) curve was used to evaluate the predictive value of plasma miRNA for myocardial injury after cardiac arrest-cardiopulmonary resuscitation in the two groups. Results The relative values of miRNA-1, miRNA-21, miRNA-126, miRNA-133a, miRNA-210, miRNA-320 and miRNA-499 in the experimental group showed statistical differences as compared with the control group in different time pionts (F = 223.037, 635.809, 51.557, 167.260, 93.157, 296.361, 622.023, all P 0.05), while there were significant differences in the rest of time points (all P 0.05), but there were significant differences in the rest of time points (all P < 0.05). However, the relative expressions of miRNA-320 and miRNA-499 at each time point had statistical differences (all P < 0.05). ROC curve analysis results showed that the areas under the ROC curve of miRNA-1, miRNA-21, miRNA-126, miRNA-133a, miRNA-320 and miRNA-499 were 0.938, 0.912, 0.859, 0.914, 0.805 and 0.969 respectively, their sensitivity were 0.750, 1.000, 0.875, 0.750, 1.000 and 0.875, and their specificity were 1.000, 0.750, 0.750, 1.000, 0.750 and 1.000 (all P < 0.05), which suggested that miRNA-1, miRNA-21, miRNA-126, miRNA-133a, miRNA-320 and miRNA-499 had predictive value for myocardial injury after cardiopulmonary resuscitation. Conclusion The relative expression of plasma miRNA of rats has different changes in myocardial injury after cardiopulmonary resuscitation, and miRNA-1, miRNA-21, miRNA-126, miRNA-133a, miRNA-320 and miRNA-499 have predictive value for myocardial injury after cardiopulmonary resuscitation. Key words: Cardiopulmonary resuscitation; Myocardial injury; MicroRNA; Rat

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