Abstract
Blueberry anthocyanins extract (BAE) is widely used natural antioxidant. The inhibitory effect of BAE (175 mg/kg bw/day) pretreatment on glycidamide (GA) metabolism following acrylamide (AA) (35 mg/kg bw/day) gavage for consecutive 7 days and 14 days in SD rat model were investigated in this work. AA, GA and its metabolites in urine, blood and 3 tissues were quantified using UPLC-MS/MS, respectively. The results showed pretreatment with BAE could significantly block the epoxidation of AA to GA. Compared with the control, BAE pretreatment significantly decreased the amount of GA and its mercapturic acid metabolite N-acetyl-S-(3-amino-2-hydroxy-3-oxopropyl)-cysteine (GAMA3) in rats urine (p < 0.05), and sharply decreased the accumulation of GA DNA adduct N7-(2-carbamoyl-2-hydroxyethyl) guanine (N7-GA-Gua) in liver, kidney and lung (p < 0.05). Meanwhile, significant reduction (p < 0.05) of N-(2-carbamoyl-2-hydroxyethyl) valine (GA-VAL) content was found in erythrocyte of the rats treated with BAE. This study provides a novel evidence for elucidating the chemopreventive effect of BAE on AA toxicity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
