Abstract
Abstract Background Posttranslational modifications have been shown to be associated with atrial fibrillation (AF). However, the relationship between ubiquitylated proteins and AF in patients with valvular heart disease remains unclear. Purpose To investigate the ubiquitination profiles in the left atrial appendage of patients with AF and valvular heart disease using LC-MS/MS analysis. Methods Eighteen subjects who underwent cardiac valvular surgery were included in this study, with nine in sinus rhythm and nine with AF. Ubiquitination profiles were analyzed using LC-MS/MS, and upregulated and downregulated ubiquitination sites were identified using quantification ratios. Results A total of 271 upregulated and 467 downregulated ubiquitination sites were identified in 162 and 156 proteins, respectively. The ubiquitylated proteins in AF were enriched in proteins associated with ribosomes, hypertrophic cardiomyopathy (HCM), glycolysis, and endocytosis. The study also revealed significant differences in the ubiquitination levels of ribosome-related and HCM-related proteins, including titin (TTN) and myosin heavy chain 6 (MYH6), between AF and sinus rhythm. Conclusions This study provides insights into the ubiquitination profiles in the left atrial appendage of patients with AF and valvular heart disease. Our findings suggest that ribosome-related and HCM-related proteins, especially TTN and MYH6, are differentially ubiquitinated in AF, which may provide a more feasible strategy against AF.Motif analysis of ubiquitylated proteinsUbiquitylated PPI network analysis
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