Abstract
Molecular genetic techniques (FISH and metabarcoding) were used to investigate comparative biodiversity in the prokaryotic complex of soil microcosms of gray forest, chestnut, and chernozem soils before and after oil pollution. At the level of high-rank taxonomic units, the structure of prokaryotic communities from different soil types was similar. In oil-polluted microcosms microbial diversity decreased, and the metbolically active dominants of the Bacteria and Archaea domains changed compared to the control samples. A specific bacterial complex was found to emerge in experimental samples of all soil types, with predominance of the Gammaproteobacteria and Actinobacteria, as well as of archaea, among which Thaumarchaeota and Crenarchaeota prevailed. Members of the soil prokaryotic complex active and inactive in respect to oil pollution were determined. Our results indicate similar succession responses of microbial communities from different soil type to oil pollution.
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