Abstract
AbstractEscherichia coli was continuously cultivated at constant dilution rate for a range of agitation rates, and at constant agitation rate over a range of dilution rates. Mean cell volume increased linearly both with increase in agitation rate (as we have previously found) and with increase in dilution rate. In each case, measurement of intracellular sodium and potassium content after rapid separation of cells from the medium by centrifugation through silicone oil showed these to increase as the mean cell volume increased. In absolute terms, the increase in sodium or potassium per unit cell volume was significantly greater for changes in agitation rate than for changes in dilution rate.
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