Changes in Composition and Content of Lipophilic Compounds in the Seedlings of <i>Triticum aestivum</i> L. Treated with Stress Phytohormones

Abstract

Exposure of plants to biotic and abiotic stress agents causes changes in the composition and content of metabolites of different chemical nature, including lipophilic compounds. One of the ways to simulate a stress situation is plant treatment with exogenous phytohormones. This work deals with investigation of organ specificity of composition of lipophilic compounds and changes in their content in wheat Triticum aestivum L. seedlings treated with exogenous stress hormones: abscisic acid (ABA), salicylic acid (SA), and methyl jasmonate (MeJA). It was found that roots and leaves of wheat seedlings have identical composition of lipophilic compounds but their content considerably differed. In the leaves, the quantity of hydrocarbons, including squalene, as well as triterpenes, sterols, and phosphatidyl choline, was much greater than in the roots. In the leaves, glycoceramides of type 1 containing a FA residue with -hydroxyl group predominated; on the contrary, glycoceramides of type 2 whose FA residues lack -hydroxyl group prevailed in the roots. Moreover, lipid extracts from the leaves contain lipophilic pigments (chlorophylls a and b and carotenoids) and hydrophobic phenolic compounds in the form of hydroxycinnamic acids. Treatment with stress phytohormones brings about considerable changes in growth characteristics, the rate of photosynthesis, and the profile of lipophilic compounds in wheat seedlings depending on the plant organ and the chemical nature of the phytohormone. In the case of ABA and MeJA, the growth of roots and leaves was suppressed, the level of nonphotochemical quenching rose, and the content of photosynthetic pigments changed. An unexpected effect was observed upon treatment with MeJA that raised the level of cholesterol and phosphatidyl serine. SA was notable for organ-specific changes in the content of products of mevalonate pathway, triterpenes, and sterols. Thus, the simulation of stress conditions by means of treatment of wheat seedlings with exogenous phytohormones strongly affected the composition of lipophilic compounds. Specific changes in lipid composition induced by hormones may contribute to adaptive structural transformations of cellular membranes, whereas changes in the content of hydrophobic phenolic metabolites and photosynthetic pigments may reinforce antioxidant defense of plants under stress conditions.