Abstract
Since the discovery in 1985 of hypervariable minisatellite regions in human DNA, “DNA fingerprinting” has been widely used in animals and plants. Although many different probes have been developed recently, most investigators still use 33.15 and 33.6 human polycore minisatellites for hybridization studies of cetaceans. This animal order shows considerably less variability than humans, and thus some limitations have to be taken into account when using these probes. The present study demonstrates that the short repetitive tandem oligonucleotide (GGAT)4 hybridizes with cetacean (Globicephala macrorhynchus andGrampus griseus) DNA, yielding individual-specific patterns of bands or “DNA fingerprints”. Freedom of design and shorter hybridization times of oligonucleotides allow an increase in the number of usable probes used, helping to overcome some of the problems found when only human polycore minisatellites are used.
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