Abstract
CE–MS can be considered a useful analytical technique for the global profiling of (highly) polar and charged metabolites in various samples. Over the past few years, significant advancements have been made in CE–MS approaches for metabolomics studies. In this paper, which is a follow‐up of a previous review paper covering the years 2012–2014 (Electrophoresis 2015, 36, 212–224), recent CE–MS strategies developed for metabolomics covering the literature from July 2014 to June 2016 are outlined. Attention will be paid to new CE–MS approaches for the profiling of anionic metabolites and the potential of SPE coupled to CE–MS is also demonstrated. Representative examples illustrate the applicability of CE–MS in the fields of biomedical, clinical, microbial, plant, and food metabolomics. A complete overview of recent CE–MS‐based metabolomics studies is given in a table, which provides information on sample type and pretreatment, capillary coatings, and MS detection mode. Finally, general conclusions and perspectives are given.
Highlights
The ultimate aim of metabolomics research is to effectively address a specific biological or clinical problem [1]
In this paper, which is a follow-up of a previous review paper covering the years 2012–2014 (Electrophoresis 2015, 36, 212–224), recent CE–MS strategies developed for metabolomics covering the literature from July 2014 to June 2016 are outlined
The largest number of metabolites was detected by RP LC–MS, while CE–MS allowed the selective analysis of amino acids in breast milk
Summary
The ultimate aim of metabolomics research is to effectively address a specific biological or clinical problem [1] To achieve this goal, state-of-the-art analytical separation techniques are increasingly used for the global profiling of (endogenous) metabolites in biological samples [2]. It is of interest to note that CE–MS has been used for the global and reproducible profiling of native peptides and (endogenous) metabolites in a clinical setting for more than a decade [7,8,9,10]. This paper, which is a follow-up of our previous CE–MS-based metabolomics reviews [21,22,23,24], provides an overview of CE–MS approaches and strategies for metabolomics studies as reported over the past 2 years. The reader is referred to more dedicated literature for an overview concerning these topics [25,26,27,28]
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