Abstract
BackgroundThe Y-box binding protein 1 (YB-1) is considered to be one of the key regulators of transcription and translation. However, so far only limited knowledge exists regarding its cellular distribution in the adult brain.ResultsAnalysis of YB-1 immunolabelling as well as double-labelling with the neuronal marker NeuN in rat brain tissue revealed a predominant neuronal expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex as well as throughout all layers of the parahippocampal cortex. In the hilus of the hippocampus single neurons expressed YB-1. The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans. Double-labelling of YB-1 with the endothelial cell marker Glut-1, the multidrug transporter P-glycoprotein, and the astrocytic marker GFAP did not indicate a co-localization. Following status epilepticus in rats, no induction of YB-1 occurred in brain capillary endothelial cells and neurons.ConclusionIn conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans. Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.
Highlights
The Y-box binding protein 1 (YB-1) is considered to be one of the key regulators of transcription and translation
YB-1 expression in the rat, macaque, and human brain Analysis of YB-1 immunolabelling in rat brain tissue revealed a strong expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex, as well as throughout all layers of the parahippocampal cortex (Fig. 1A–D)
This analysis confirmed a co-localization of YB-1 and neuron-specific nuclear antigen (NeuN) (Fig. 2A–B)
Summary
The Y-box binding protein 1 (YB-1) is considered to be one of the key regulators of transcription and translation. Y-box binding proteins constitute a family of DNA/RNAbinding proteins that control gene expression at both the transcriptional and translational level [1,2]. The Y-box protein 1 (YB-1) represents a major mRNP (ribonucleoprotein particle) protein modulating the overall structure of mRNA to regulate translation [3]. With this function, YB-1 represents one of the key regulators for expression of the multidrug transporter P-glycoprotein (Pgp) in tumor cells [4,5,6,7,8,9,10]. Several studies confirmed that high YB-1 expression levels are associated with tumor aggressiveness, failure of chemotherapy, and a poor prognosis [13,14,15,16]
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