Abstract
Cell survival and plasmid stability in Pseudomonas syringae pv. mori Ni27 containing RSF1010 or its derivative recombinant plasmids (newly constructed pNIA1, pNIA2) were studied in soil and culture media. There was no apparent difference in survival-rate in sterile or non-sterile soil between plasmid-bearing strains and plasmid-free strain. Plasmid stability was strongly influenced by soil conditions (sterile or non-sterile/incubation temperature) and plasmid type in soil, and it was influenced by some culture conditions (temperature/slant or liquid/shaking or still/kind of medium) in culture media. Especially a low temperature (5°C) treatment was effective to lose plasmids in the broth still culture and in sterile soil.
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