Abstract
Mouse diploid and tetraploid H-1 (ES) cells cultured in L15F10 medium (2nH1 and 4nH1 cells, respectively) without leukemia inhibitory factor (LIF) (2nH1(−) and 4nH1(−) cells, respectively) were examined for their growth and cellular characteristics. Though the doubling times of 2nH1(−) and 4nH1(−) cells were longer than those of 2nH1 and 4nH1 cells, the phase fractions of 2nH1(−) and 4nH1(−) cells were the same as those of 2nH1 and 4nH1 cells, suggesting that occasional cell deaths occurred. DNA loss in long-term culturing was observed in 4nH1(−) cells, but not in 2nH1(−) cells, suggesting that DNA decay was independent of LIF. The cell volume, cellular morphology, and the pluripotent potential of 2nH1(−) and 4nH1(−) cells were retained during long-term culturing, suggesting that 2nH1(−) and 4nH1(−) cells survived in the medium without LIF had similar characteristics to those of 2nH1 and 4nH1 cells, respectively.
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