Abstract
Methods First, we have developed cryopreservation methods that allow infection of foreskin tissue explants after thawing (654 +/178.3 pg of p24/gr of tissue) and provide comparable infection rates to fresh samples( 716.5 +/446pg of p24/gr of tissue). Second, we have developed an ex vivo assay that uses human foreskin explants to replicate the polarized viral entry. After 12h of polarized exposure, HIV entering the explant is amplified for 6 days of culture with activated PBMCs and measured using p24 ELISA.
Highlights
In sexually insertive men, HIV is predominantly transmitted at the penile surface
We report two mayor advances in the study of HIV infections at the foreskin
First, we have developed cryopreservation methods that allow infection of foreskin tissue explants after thawing (654 +/- 178.3 pg of p24/gr of tissue) and provide comparable infection rates to fresh samples( 716.5 +/- 446pg of p24/gr of tissue)
Summary
HIV is predominantly transmitted at the penile surface. We report two mayor advances in the study of HIV infections at the foreskin. Cell free HIV-1 virus can infect inner and outer foreskin polarized explants MP Lemos1*, ML Perez1, J Sanchez2, J Lama2, S Montano3, J McElrath1 From AIDS Vaccine 2012 Boston, MA, USA.
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