Abstract
We report on cell damage of single cells confined in continuous-wave (cw), near-infrared (NIR) multimode optical traps as a result of multiphoton absorption phenomena. Trapping beams at NIR wavelengths less than 800 nm are capable of damaging cells through a two-photon absorption process. Cell damage is more pronounced in multimode cw traps compared with single-frequency true cw NIR traps because of transient power enhancement by longitudinal mode beating. Partial mode locking in tunable cw Ti:sapphire lasers used as trapping beam sources can produce unstable subnanosecond pulses at certain wavelengths that amplify multiphoton absorption effects significantly. We recommend the use of single-frequency long-wavelength NIR trapping beams for optical micromanipulation of vital cells.
Highlights
A novel micromanipulation tool in vital cell biology is optical traps
With the increased availability of tunable Ti:sapphire and compact diode laser sources, there has been substantial interest in developing optical traps at shorter NIR wavelengths, at which water absorption can drop by as much as a factor of 10.3 This can be beneficial in the case of motile cell studies in which it is essential to produce trapping forces that are higher than the adenosine triphosphate –driven intrinsic motility forces
Letter we provide evidence that cell damage occurs in single-frequency traps at NIR wavelengths of,800 nm and that trap-induced cell damage is more pronounced in multimode cw traps
Summary
A novel micromanipulation tool in vital cell biology is optical traps. Single-beam gradient force traps are created when cw laser beams are focused with an objective of high numerical aperture (NA). Title Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption. Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption
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