Abstract
This work is carried out to determine the empirical boundary conditions of concentration/separation of yeast cells, red blood cells of rats and guinea pigs in standing ultrasonic wave, based on the performed theoretical calculations. The results indicated that increase flow rate of the medium above the theoretically calculated values of cell retention in a standing ultrasonic wave causes them to wash away from the camera separation ultrasound.
Highlights
In the past few years, more and more ultrasonic methods are employed as filters in medicine and biotechnology
The results indicated that increase flow rate of the medium above the theoretically calculated values of cell retention in a standing ultrasonic wave causes them to wash away from the camera separation ultrasound
We investigated these two forces, and picked up the boundary conditions for the concentration and separation of cells in suspension in a standing ultrasonic wave, which allowed us to theoretically find the flow rate environment and the average energy density
Summary
In the past few years, more and more ultrasonic methods are employed as filters in medicine and biotechnology. Filters of this type are able to effectively delay the different particles (for example, cells) in variable-pressure nodes. There are many concepts on the separation and concentration of suspended particles of different origin under the forces acting on these particles in ultrasonic fields. They can be classified into categories based on the use of 1) standing or running acoustic waves; 2) modes with continuous and periodically stopped medium flows; and 3) resonant or non-resonant conditions. Other forces can be used, depending on the design of the device intended for ultrasonic selection
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