Abstract
1. 1. Cytidine diphosphodiacyl sn-glycsrol: inositoltransferase activity was located preponderantly in the microsomal fraction of rabbit mammary tissue. 2. 2. Dialysis against potassium phosphate buffer and the inclusion of mercaptoethanol 5 mM in assays increased specific activity of microsomes from 1.5 to 4.5 and 8.0 nmoles phosphatidylinositol formed per mg protein/min, respectively. 3. 3. Manganese was required and 6 mM was optimum. Enzyme activity with CDP-didecanoyl sn-glycerol was twice that obtained with CDP-dioleyl sn-glycerol. K m values of 40 μM and 250 μM were obtained for CDP-didecanoyl sn-glycerol and inositol, respectively. 4. 4. During pregnancy the total mammary activity of CDP-diacyl sn-glycerol:inositoltransferase increased 10-fold between day 20 and day 26. After parturition there was an additional 4-fold increase in activity. At peak lactation the mammary tissue isolated from a single rabbit had a capacity to synthesize 4–5 m-moles phosphatidylinositol per day.
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