CDNA cloning, sequencing and in situ localization of a transcript specific to both sublingual demilune cells and parotid intercalated duct cells in mouse salivary glands

Abstract

A cDNA clone derived from mouse sublingual gland was isolated from a λ-phage cDNA library. Northern blot hybridization indicated that the transcript from which it was derived was approx. 700 nucleotides in length. This mRNA encoded a protein of about 20 kDa, as determined by hybrid selection and cell-free translation. Conceptual translation of the cDNA clones showed that p20 is 170 amino acids in length. The putative protein is hydrophobic in nature, is neither a mucin-like protein nor does its amino acid sequence or composition resemble the other known mouse proteins. However, the amino acid sequence of p20 suggests that it may be from a gene or gene family homologous to rat common salivary protein 1. The p20 mRNA also appears to share a non-random degree of sequence homology with the cysteine-rich domains of bovine and porcine submandibular mucins. The p20 mRNA is abundant in the mouse sublingual gland, and its expression is approx. nine times greater than in the parotid gland. In situ hybridizations localized the p20 mRNA exclusively in the demilune cells of the sublingual gland and in the intercalated duct cells of the parotid gland. It is detectable in the neonatal and adult submandibular gland at very low levels, but is absent from liver, heart, brain, thymus, spleen, lens and lacrimal glands.