Abstract
Angelica sinensis (Oliv.) Diels (Umbelliferae) is a well-known medicinal plant mainly distributed in Gansu Province of China. Its local and global demand is significant because of its food and medicinal applications. However, the early bolting rate of Angelica sinensis (Oliv.) Diels reaches 20%–60%, which seriously affects its food and medicinal qualities. Thus, differences in gene expression between the flower bud and sprout-shoot apical meristem underwent analysis, by means of cDNA-amplified restriction fragment length polymorphism, to better understand the flowering mechanism. 64 primer sets, each of which amplified to 60 transcript-derived fragments (TDFs), were used. Among these TDFs, 26 were expressed specifically in the flower bud. After cloning and sequencing, 32 distinct sequences were obtained from these 26 TDFs, and 25 were found with homologous sequences in databases. Confirmation of differential expression of 13 sequences was obtained by semi-quantitative RT-PCR, their showing higher expression levels in flower buds. These homologous sequences encode transposable elements, pentatricopeptide repeat-containing proteins, DNA-binding transcription factors, zinc finger (B-box type) family proteins, NADP-dependent sorbitol 6-phosphate dehydrogenase (S6PDH), amongst others.
Highlights
Angelica sinensis (Oliv.) Diels (Umbelliferae) is a world-famous medicinal plant distributed throughout Gansu Province, China
In Gansu Province where it originated, the quality of Angelica sinensis has been seriously affected by its high early bolting rate
Emphasis was placed on analyzing gene expression differences between the flower bud and sprout-shoot apical meristem, as well as on identifying certain key genes regulating bolting in Angelica sinensis
Summary
2.9 family) zinc finger (C3HC4-type RING finger) family protein / ankyrin repeat family protein. HO056136 HO056138 HO056139 retrotransposon protein Prunus umbellata NADP-dependent sorbitol 6-phosphate dehydrogenase (s6pdh) gene serine /threonine kinase-like protein ATAF-like NAC-domain transcription factor putative reverse transcriptase transposable element gene; non-LTR retrotransposon family (LINE) transposable element gene , pseudogene, hypothetical protein photosystem II protein Z [Daucus carota]protein transposable element gene; copia-like retrotransposon family
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