CD4 + lymphocytes require platelets for adhesion to immobilized fibronectin in flow: Role of β 1 (CD29)-, β 2 (CD18)-related integrins and non-integrin receptors

Abstract

The role of platelets in T-lymphocytes adhesion is not clear yet. Herpesvirus saimiri (HVS)-infected CD4 + T-lymphocytes were placed into polystyrene plates pre-coated with fibronectin. The adherent T-cells were enumerated by image analysis. Under static condition, 38 ± 10 cells/mm 2 adhered and addition of gel-filtered platelets (GFP) and PMA enhanced cell adhesion 4.3- and 4.1-fold. Using PMA plus GFP 11.9-fold enhancement in cell adhesion was achieved. In contrast, under flow (200 s −1), neither basal adhesion nor following separate addition of PMA or GFP was observed, whereas combined addition of PMA and GFP induced noticeable adhesion (34 cells/mm 2). The adhesion was inhibited by blockade of α 5-integrin (CD49e, 87%), β 2-integrin (CD18, 78%), CD40L (60%), PSGL-1 (CD162, 60%), and CD40L plus PSGL-1 (83%). Thus, activated platelets promote activated T-cell adhesion to fibronectin under flow via integrins (α 5β 1, and α Lβ 2), CD40–CD40L and P-selectin–PSGL-1 mediated interactions.