Abstract
BackgroundThe biological effects of CD24 (FL-80) cross-linking on breast cancer cells have not yet been established. We examined the impact of CD24 cross-linking on human breast cancer cell line MCF-7.MethodsMCF-7 and MDA-MB-231 cells were treated with anti-rabbit polyclonal IgG or anti-human CD24 rabbit polyclonal antibodies to induce cross-linking, and then growth was studied. Changes in cell characteristics such as cell cycle modulation, cell death, survival in three-dimensional cultures, adhesion, and migration ability were assayed after CD24 cross-linking in MCF-7.ResultsExpression of CD24 was analyzed by flow cytometry in MDA-MB-231 and MCF-7 cells where 2% and 66% expression frequencies were observed, respectively. CD24 cross-linking resulted in time-dependent proliferation reduction in MCF-7 cells, but no reduction in MDA-MB-231 cells. MCF-7 cell survival was reduced by 15% in three-dimensional culture after CD24 cross-linking. Increased MCF-7 cell apoptosis was observed after CD24 cross-linking, but no cell cycle arrest was observed in that condition. The migration capacity of MCF-7 cells was diminished by 30% after CD24 cross-linking.ConclusionOur results showed that CD24 cross-linking induced apoptosis and inhibited migration in MCF-7 breast cancer cells. We conclude that CD24 may be considered as a novel therapeutic target for breast cancer.
Highlights
The biological effects of CD24 (FL-80) cross-linking on breast cancer cells have not yet been established
CD24 expression in MDA-MB-231 and MCF-7 cells Expression of CD24 was analyzed in MDA-MB-231 and MCF-7 cells by flow cytometry, which revealed that 2% of MDA-MB-231 cells and 66% of MCF-7 cells expressed CD24 (Fig. 1)
We selected MDA-MB231 as negative control and MCF-7 as an experimental cell line for examining the effect of CD24 cross-linking on the growth of breast cancer cells
Summary
The biological effects of CD24 (FL-80) cross-linking on breast cancer cells have not yet been established. We examined the impact of CD24 cross-linking on human breast cancer cell line MCF-7. Accumulating evidence supports a role for CD24 in a variety of malignancies, including B-cell lymphoma, renal cell carcinoma, small-cell and non small-cell lung carcinoma, nasopharyngeal carcinoma, hepatocellular carcinoma, bladder carcinoma, epithelial ovarian cancer and breast cancer [3]. Important role in the progression and metastasis of human breast cancer [8,18]. The aim of this study was to further clarify the role of CD24 in breast cancer cell growth using a cross-linking approach. We did to study directly impact on cross-linking with CD24 (FL-80) antibody in MCF-7 human breast cancer cell line. CD24 has several potential N- and O-linked glycosylation sites, which act as ligands for P-selectin [4]
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