Abstract
The neo sequences in pMCI Neo-polyA end several bases after the stop codon and are followed by a short sequence derived from the herpes simplex virus tk gene, which carries two tandem polyA signals. These sequences probably stimulate the efficiency of splicing from this site by improving the pyrimidine content compared with the natural Tn5 sequences. Because we and at least one other group (Ref. 4) have observed the use of this cryptic exon, it is important when planning a targeting strategy using the neo gene to be aware of this possibility, particularly because this exon contains an open reading frame.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
