Cattle Theileriosis: Prevalence and Comparative by Using ELISA and Blood Smears Techniques in Sulaimani Province - Kurdistan Region / Iraq.

Abstract

Isolated one hundred fifty adult female cattle (local breed) one hundred infested with a hard tick (Ixodidae) and show some clinical signs for a search of theileriosis such as; enlargement pre-scapular lymph node and pale of the eye mucus membrane, fifty of them use a control (Non infested cattle) during the period of April to November 2018 in Sulaimani province. Indicated the highest sensitivity (prevalence) of Theileria annulata when examined by ELISA technique 63% (63/100), the prevalence rate of macroscopically detection to T. annulata merozoite was 48% as the following, in mixed lymph node biopsy cells and blood smears technique was 22%, the blood and lymph node biopsy smear examinations were 15% and 11% respectively. Distinguished the total prevalence rate in control cattle was 6% and 4% in the ELISA technique and microscopically method respectively. Sensitivity values and positive predictive noted a highly in ELISA serolog-ical test 63% and 95.45% respectively, while in mixed lymph node biopsy and blood smears examined 22% and 100% respectively. During the study collected 1368 hard tick and identified to three genera in infested cattle of Ixodidae; Hyalomma species are the highest number percentage (44.8%), followed Boophilus species (28.6%) and the Rhipicephalus species is the lowest percentage (26.6%). This study conducted to find out the prevalence the local cattle with theileriosis (T. annulata) by the microscopic examination for both blood and lymph biopsy smear (merozoite) and compared with the (ELISA) technique.