Cassia mimosoides acute toxicity and D-galactosamine induced liver injury mice

Abstract

Objective To observe the acute toxicity reaction of Cassia mimosoides and to observe the influence of Cassia mimosoides decoction on the levels of ALT and AST of liver injury mice.Methods Cassia mimosoides was given a maximum dose to observe the acute toxicity reaction of drugs.The mice were randomly divided into high and low dose groups,a positive control group,a model control group and a normal control group.Besides the normal control group,D-GalN was intraperitoneally injected in all the other groups to make liver injury models.Continuous administration lasted for 5 days,1 times per day.1 hour after the last administration,the serum ALT and AST levels were determined.Results The maximum tolerance dose of mice was 150 g/kg,equivalent to 500 times of clinical medicine; the animal were generally in good condition in this dose; no obvious symptoms of toxicity was found.There were statistical differences in AST and ALT levels between the high dose group and the model group.Though there were no statistical differences in AST and ALT levels between the low dose group and the model group,there was a downward trend in AST an ALT levels.Conclusions Mice taking a dose less than maximum tolerance dose has no acute toxicity.Cassia mimosoides decoction can decrease the levels of AST and ALT of liver injury mice,suggesting that Cassia mimosoides decoction has a protective effect on liver injury.High dose group was superior to low dose group,showing that the protective effect increases with dose.Its mechanisms need to be further explored and studied. Key words: Cassia mimosoides; Liver injury; D-galactosamine; ALT; AST