Abstract
In this paper, carbon nanotubes–hydroxyapatite (MWCNTs–HA) composite were synthesized by self-assembling technique, and investigated by X-ray diffraction, scanning electron microscopy (SEM) and FT-IR spectroscopy. Hemoglobin (Hb) immobilized in MWCNTs–HA film not only retained its similarly native conformations, but also achieved the direct electron transfer. The apparent heterogeneous electron transfer rate constant ( k s) was evaluated as (5.05 ± 0.41) s − 1 according to Laviron's equation, and the surface coverage ( Γ*) was estimated as (9.25 ± 0.69) × 10 − 10 mol cm − 2 . Moreover, the Hb immobilized in MWCNTs–HA film exhibited remarkable bioelectrocatalytic activity for the electrochemical reduction of H 2O 2. The amperometric response of the biosensor varied linearly with the H 2O 2 concentration ranging from 0.5 μM to 2 μM, and the results showed a detection limit of 0.09 μM. Furthermore, the Hb-immobilized MWCNTs–HA film exhibited the excellent catalytic reactivity toward trichloroacetic acid (TCA).
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