Carbon Dots Prepared by One-step Sintering Method and their Application for pH Detection

Cigarette smoke is one of the six major pollution sources in the room air. It contains large number of particles with size less than 10 nm. There exist carbon dots (CDs) in cigarette smoke which have strong fluorescence and with good bio-compatibility and low toxicity. CDs in cigarette smoke can be applied in bio-imaging which has great potential applications in the integration of cancer diagnosis and treatment. In this paper, CDs were extracted from cigarette smoke. Then, sodium borohydride was added to CDs aqueous solution for reduction and the reduced CDs (R-CDs) were used for biological cell imaging. The results indicate that the CDs with the particle size <10 nm in cigarette smoke are self-assembled by the polymerizated polycyclic aromatic hydrocarbons (PAHs) and ammonium nitrite which are disk nano-structure composed of sp2/sp3 carbon and oxygen/nitrogen groups or polymers. Sodium borohydride can reduce the carbonyl group on the surface of CDs to hydroxyl group and increase the ratio of the Na 1s ratio of the CDs from 1.86 to 7.42. The CDs can emit blue fluorescence under ultraviolet irradiation. After reduction, the R-CDS have the intensity of fluorescence 7.2 times than before and the fluorescence quantum yield increase from 6.13% to 8.86%. The photoluminescence (PL) wavelength of R-CDS have red-shift of 7 nm which was due to the increasing of Na element ratio. The onion epidermal cells labeled with R-CDs show that the CDs could pass through the cell wall into the cell and reach the nucleus. The cell wall and the nucleus could be clearly visualized. CDs also shows low toxicity to human bronchial epithelial cells (BEAS-2B) with good biological activity. The obtained results indicate that the CDs and R-CDs have good fluorescent property which could be used as bio-imaging agent.

Microwave enhanced carbon dots synthesis from eggshell membrane: Versatile applications in heavy metal ion sensing, strain free detection of fingerprints, UV shielding, food packing and anti-counterfeiting

Reparation of porous Ti-Cu alloy by one-step sintering method and application of hydrogen evolution reaction

Enhanced photoluminescence properties of a carbon dot system through surface interaction with polymeric nanoparticles

Ultra small carbon dots from Crocus cancellatus as a highly fluorescence probe for synthetic Ponceau 4R dye sensing in food samples

A novel approach to prepare high density SiC ceramics by powder extrusion printing (PEP) combined with one-step sintering method

Facile synthesis of fluorescent carbon dots from Prunus cerasifera fruits for fluorescent ink, Fe3+ ion detection and cell imaging

SummaryProtons are highly related to cell viability during physiological and pathological processes. Developing new probes to monitor the pH variation could be extremely helpful to understand the viability of cells and the cell death study. Carbonized polymer dots (CPDs) are superior biocompatible and have been widely applied in bioimaging field. Herein, a new type of extreme-pH suitable CPDs was prepared from citric acid and o-phenylenediamine (CA/oPD-CPDs). Due to the co-existence of hydrophilic and hydrophobic groups, CA/oPD-CPDs tend to aggregate in neutral condition with a dramatic decrease of fluorescence, but disperse well in both acidic and alkaline conditions with brighter emission. This specialty enables them to selectively illuminate lysosomes in cells. Moreover, CA/oPD-CPDs in the cytoplasm could serve as a sustained probe to record intracellular pH variation during apoptosis. Furthermore, CA/oPD-CPDs present a continuous fluorescence increase upon 2-h laser irradiation in living cells, underscoring this imaging system for long-term biological recording.

Functional metal doping endows fluorescent carbon dots with richer physical and chemical properties, greatly expanding their potential in the biomedical field. Nonetheless, fabricating carbon dots with integrated functionality for diagnostic and therapeutic modalities remains challenging. Herein, we develop a simple strategy to prepare Gd/Ru bimetallic doped fluorescent carbon dots (Gd/Ru-CDs) via a one-step microwave-assisted method with Ru(dcbpy)3Cl2, citric acid, polyethyleneimine, and GdCl3 as precursors. Multiple techniques were employed to characterize the morphology and properties of the obtained carbon dots. The Gd/Ru-CDs are high mono-dispersity, uniform spherical nanoparticles with an average diameter of 4.2 nm. Moreover, X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FTIR) confirmed the composition and surface properties of the carbon dots. In particular, the successful doping of Gd/Ru enables the carbon dots not only show considerable magnetic resonance imaging (MRI) performance but also obtain better fluorescence (FL) properties, especially in the red emission area. More impressively, it has low cytotoxicity, excellent biocompatibility, and efficient reactive oxygen species (ROS) generation ability, making it an effective imaging-guided tumor treatment reagent. In vivo experiments have revealed that Gd/Ru-CDs can achieve light-induced tumor suppression and non-invasive fluorescence/magnetic resonance bimodal imaging reagents to monitor the treatment process of mouse tumor models. Thus, this simple and efficient carbon dot manufacturing strategy by doping functional metals has expanded avenues for the development and application of multifunctional all-in-one theranostics.

Carbon dots (CDs) have outstanding optical properties, biocompatibility, and photostability, making them attractive for imaging applications. A facile and green one-step hydrothermal synthesis method is proposed, which can be safely used in a wide range of applications such as chemical sensing, bioimaging, and optoelectronics. In this study, we report green synthesis of carbon dots from bitter orange juice (Citrus Aurantium) by hydrothermal treatment for the first time. We studied effects of time, temperature, and pH on fluorescence of CDs, characterized them using various spectroscopic and microscopic methods, and evaluated their toxicity to different cell lines. Identifying an optimum reaction condition of 180 ºC for 7 h heating gave CDs that showed pH-dependent fluorescence, with the largest fluorescence at a pH of 7.0. The CDs were 1-2 nm in size with a spherical morphology and negative surface charge. The CDs showed a high quantum yield of 19.9 %, reasonable photostability, excellent water solubility, and long fluorescence lifetime. A one step hydrothermal rout led to various hydrophilic functional groups on the surface of the CDs. Our results showed that the CDs were non-toxic over a large concentration range and effective for imaging of cells, indicating their potential as imaging probes in medical diagnostics and biosensor applications.

Herein, we have synthesized carbon dots (CDs) using a one-step hydrothermal method from green tea waste, a biomass-derived source with high fluorescent properties and excellent solubility in water. The synthesis of CDs was confirmed through a comprehensive range of characterization techniques, including HRTEM (high-resolution transmission electron microscopy), XPS (X-ray photoelectron spectroscopy), and EDX (energy-dispersive X-ray spectroscopy). The optical properties of the synthesized CDs were assessed using UV-Vis spectroscopy and fluorescence (FL) spectroscopy. The CDs displayed exceptional stability across a wide pH range and various concentrations. Moreover, these CDs exhibited a photoluminescence quantum yield (PLQY) of 21.6%, indicating their efficiency in emitting fluorescent light upon excitation. The CDs also showcased their prowess in fluorometrically detecting Cu2+ ions, displaying high sensitivity and selectivity. They presented two distinct linear ranges: 0.02 to 50µM and 50 to 100µM, with recovery rates ranging from 94.2 to 104.06%. Moreover, under visible light irradiation, the CDs exhibited significant efficiency in the photocatalytic removal of dyes. Specifically, the CDs achieved degradation rate of 97.89% for Rhodamine B (RhB) within a 30-min irradiation period. In the context of RhB adsorption, it is evident that the experimental data align more closely with the Freundlich isotherm than the Langmuir isotherm. This is substantiated by a higher R2 value (0.97) for the Freundlich isotherm model compared to the Langmuir adsorption isotherm model (0.93). Notably, the adsorption kinetics was effectively described by pseudo first-order kinetics models. Overall, these results highlight the promising potential of CDs in applications such as environmental remediation and waste treatment processes due to their photocatalytic and sensing capabilities.

Excessive urea in milk will lead to serious health problems. To detect whether the urea concentration in milk exceeds the standard and ensure the quality of milk, it is necessary to develop detection technology for urea in milk. But it is difficult to detect urea in milk conveniently and accurately by traditional methods. To measure the concentration of urea in milk, stable green light carbon dots (CDs) were synthesized by a one-step method as a fluorescent probe. Then, 3, 5-diaminobenzoic acid was used as the precursor for CD synthesis. Experimental results showed that CDs can generate strong fluorescence when excited by light (350-450 nm). The fluorescence peak wavelength is 490 nm, and the optimum excitation wavelength is 390 nm. The fluorescence intensity of CDs has a significant change with variations of pH (pH of 6-9), and the higher the pH, the lower the fluorescence intensity. Additionally, urea can be hydrolyzed by urease to produce ammonia and carbon dioxide. Ammonia is ionized in water to produce OH-, which increases the pH of the solution. After adding standard urea to milk, urease and CDs are added. The fluorescence intensity of CDs in the mixed solution decreases as the concentration of standard added urea increases. Thus the concentration of urea in milk can be calculated. The experimental results show that the CD method for detecting urea in milk has advantages of high sensitivity and wide measurement range. The linear interval is 25-500 mg/L, R2 is 0.998, and the limit of detection is 6.27 mg/L. The concentration of urea in the milk used in the experiment is 265.46 mg/L. CDs are easy to fabricate, and the advantages of the method are simple operation, no pretreatment, safety, and low cost. A new method for the detection of urea in milk was established, to the best of our knowledge, and this method can aid in food quality control.

Constructing ultra-stable photothermal plastics assisted by carbon dots with photocaged reactivity

Preparation of highly photoluminescent carbon dots from polyurethane: Optimization using response surface methodology and selective detection of silver (I) ion

As a new type of carbon nanomaterials, carbon dots have attracted great interest due to their non-toxic, low preparation cost and unique photoluminescence properties. They have been widely studied and shown great potential in many fields. Here, nitrogen doped fluorescent carbon dots were prepared by one-step hydrothermal method using Epiphyllum leaves as raw materials. Ultraviolet-visible absorption spectrometer and fluorescence spectrometer were used to study the optical properties of carbon dots. The elemental composition and morphology of the surface of carbon dots were analyzed by transmission electron microscope, Fourier transform infrared spectroscopy and X-ray photoelectron spectrometer. The results show that the prepared carbon dots are uniformly dispersed in the aqueous solution, emit blue fluorescence, and the average particle size is 4.2 nm. The carbon dots have good light stability and strong anti-photobleaching ability. Continuous spectral scanning of the carbon dots can keep the fluorescence intensity basically unchanged within 30 minutes. In the concentration of 0∼f molL-1 NaCl solution, the strength is not affected by the ionic strength. In the pH range of 4∼ 8, the fluorescence intensity of carbon dots is basically stable. The heavy metal ion pb2+ has a quenching effect on the fluorescence of carbon dots, and has a good linear relationship in the range of 0.5*200 μm. Therefore, a simple and green method for pb2+ has been developed.