Capture and toxicity assessment of Ag citrate nanoparticles using jellyfish extract

Abstract

In this study, Jellyfish extract at immunity reaction (JEI) captured Ag citrate nanoparticles (Ag citrate NPs) and reduce toxicity was investigated. JEI obtained from Aurelia aurita was used to assess the capture of Ag citrate NPs (10 mg/L, 2 mg/L) during 24 h, and the toxicity of JEI was assessed using a human cell line BEAS-2B. JEI was to reduce Ag citrate NPs in water by 34.55% (10 mg/L) after 1 h and by 40.62 ~ 41% (2 mg/L, 10 mg/L) after 24 h. When the BEAS-2B was exposed to 2 mg/L of Ag citrate NPs mixed with JEI showed increase 33.97% survival than 2 mg/L of Ag citrate NPs (0%). Furthermore, the apoptosis-related genes (p53, Bcl2, and Bax) showed no significant difference in expression upon exposure to JEI, compared to the control. JEI is to capture Ag citrate NPs and reduce its nano-toxicity. Therefore, such ability of JEI is likely to prove useful in the control of AgNPs flowing into the aquatic ecosystem.