Abstract
Abstract The link between brain and reproduction is the release of the decapeptide luteinizing hormone-releasing hormone (LHRH) from the brain median eminence (ME), a release modulated by other neuropeptides such as β-endorphine (BEND) and neuropeptide Y (NPY) impinging on the LHRH neuron. Analysis of brain perfusates is the metnod of choice to determine in vivo release from discrete brain areas. For this, push-pull cannula (PPC) and microdialysis sampling are the most prominent technologies. However, sample volume is a limiting issue when attempting to measure by radioimmunoassays (RIA) in vivo release of multiple neuropeptides from these sources, especially when neuropeptide concentration is low and the whole sample (100 μl PPC perfusate/10 min) has to be dedicated to measure only one neuropeptide. We developed a capillary electrophoresis (CE)-based assay for the simultaneous determination of LHRH, BEND and NPY in PPC samples from the ewe ME. This assay uses CE for the separation of these neuropeptides ...
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