Abstract
Background: Hemp (Cannabis sativa L.) seed contains high contents of various nutrients, including fatty acids and proteins. Cannabidiol (CBD) is a non-psychoactive compound that can be extracted from C. sativa and used for treating epilepsy and pain. Industrial hemp products, including CBD and hemp seed oils, have become increasingly popular. Some products are marketed without a clear distinction between CBD and hemp seed oils. Herein, the CBD content and biological activities of commercial CBD and hemp seed oils were examined. Methods: CBD content was measured by high-performance liquid chromatography. For in vitro antioxidant activity determination, 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging assays were performed. Results: The CBD concentrations in the two CBD oil samples were 18.9 ± 0.5 and 9.2 ± 0.4 mg/mL. Of the seven hemp seed oil samples, six samples contained CBD in concentrations ranging from 2.0 ± 0.1 to 20.5 ± 0.5 µg/mL, but it was not detected in one sample. Antioxidant activity was observed in both CBD oil samples. Conclusions: The results indicate that (1) CBD content varied by hemp seed oil sample and that (2) antioxidant activity could be a useful landmark for discriminating CBD oils from hemp seed oils.
Highlights
Cannabis sativa L. has been cultivated worldwide for centuries for medicinal, industrial and recreational use
Two CBD and seven hemp seed oil samples were evaluated, while rapeseed oil was used as a CBD-free sample
Because CBD exists primarily in the carboxylated form, cannabidiol acid (CBDA), in fresh C. sativa L. plants, hemp seed oil samples were heated at 100 ◦ C for 2 h to decarboxylate CBDA into CBD [12]
Summary
Cannabis sativa L. has been cultivated worldwide for centuries for medicinal, industrial and recreational use. Commercial CBD products are primarily produced from industrial C. sativa or hemp, which contains little or no THC [9,10]. Some reports have demonstrated that cannabinoids can be detected in commercial hemp seed products [12,13]. On the existence of CBD in hemp seed oils commercially available in Japan. Some products are marketed without a clear distinction between CBD and hemp seed oils. Commercially available CBD and hemp seed oil products were analyzed using high-performance liquid chromatography (HPLC).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
