Abstract
Anthracnose, caused by Colletotrichum lindemuthianum, is an important fungal disease of common bean (Phaseolus vulgaris). Alleles at the Co–4 locus confer resistance to a number of races of C. lindemuthianum. A population of 94 F4:5 recombinant inbred lines of a cross between resistant black bean genotype B09197 and susceptible navy bean cultivar Nautica was used to identify markers associated with resistance in bean chromosome 8 (Pv08) where Co–4 is localized. Three SCAR markers with known linkage to Co–4 and a panel of single nucleotide markers were used for genotyping. A refined physical region on Pv08 with significant association with anthracnose resistance identified by markers was used in BLAST searches with the genomic sequence of common bean accession G19833. Thirty two unique annotated candidate genes were identified that spanned a physical region of 936.46 kb. A majority of the annotated genes identified had functional similarity to leucine rich repeats/receptor like kinase domains. Three annotated genes had similarity to 1, 3-β-glucanase domains. There were sequence similarities between some of the annotated genes found in the study and the genes associated with phosphoinositide-specific phosphilipases C associated with Co-x and the COK–4 loci found in previous studies. It is possible that the Co–4 locus is structured as a group of genes with functional domains dominated by protein tyrosine kinase along with leucine rich repeats/nucleotide binding site, phosphilipases C as well as β-glucanases.
Highlights
Common bean (Phaseolus vulgaris L.) with a diploid genome (2n = 2x = 22) is among the most important food legumes used for direct human consumption [1]
It is possible that the Co–4 locus is structured as a group of genes with functional domains dominated by protein tyrosine kinase along with leucine rich repeats/nucleotide binding site, phosphilipases C as well as β-glucanases
The distribution pattern of the anthracnose resistance rating among the entries used is included in S1 Fig. Out of the 5361 markers used for genotyping, only 538 showed polymorphisms and were used for mapping in the 94 F4:5 recombinant inbred lines (RILs) population
Summary
Common bean (Phaseolus vulgaris L.) with a diploid genome (2n = 2x = 22) is among the most important food legumes used for direct human consumption [1]. Among the environmental factors that affect bean production, losses caused by fungal diseases can be drastic and have high importance. There are a number of important biotic stresses in common bean, anthracnose, caused by the hemibiotrophic fungus Colletotrichum lindemuthianum is one of the globally important fungal pathogens affecting sustainability of production [2]. With the adoption of no-tillage or limited tillage faming systems that increase the amount of debris hosting the pathogen to remain in the field, it is likely that anthracnose will gain more importance in the future
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