Abstract
Introduction: The objective of this study was to compare the stability properties of the two years for the quality of Tri-Pha-La Herbal Drink, Thai traditional and Ayurvedic medicine. This study aimed to compare the anticancer and anti-HIV efficacy of the solvent extracts and gallic acid of the three fruits. The research was done between A.D. 2017 to A.D. 2018.
 Materials and Methods: In the present study, the cancer cell lines, KB, HT 29, MCF-7, A 549, CL and ASK treated with Triphala fruits extract at concentrations of 4 mg/mL for 72 h except 48 for P-388) . The cytotoxic activity was expressed as 50% effective dose (ED50). In addition, anti-HIV activity of the all Triphala fruits solvent extracts was performed by anti-HIVs-1RT. The tested extracts were prescreened at 200 μg/mL and only those which were very active (≥70% inhibition) at this concentration were further determined for the dose, μM, that inhibited 50% HIV-1 RT activity (IC50). Moreover, anti-syncytium (MC99+1A2) was performed by the Triphala fruits extract. The results were expressed as the concentration that inhibited 50% formazan formation in uninfected cells (IC50). The therapeutic index (TI) was calculated using the equation: TI=IC50/EC50.
 Results: The results were compared between years A.D. 2017/2018. Amongst six ethyl acetate and methanol extracts (A.D. 2017) showed less than 20 µg/mL cell viability in P-388 cell lines. However, the four ethyl acetate and methanol extracts (A.D. 2018) were also inhibited P-388 cell species. Furthermore, each gallic acid, isolated from three fruits Triphala (A.D.2017/2018) were also strongly inhibited to P-388 cell species. Anti-HIV-RT activity of the all Triphala fruits solvent extracts (A.D. 2017) except hexane extracts showed very active which is consistent with A.D. 2018.
 Conclusion: P. emblica-ethyl acetate extract (A.D. 2018) was the most promising anti-syncytium (MC99+1A2) among evaluated all three fruits Triphala extracts: it afforded the lowest EC ₅₀ 8.85 µM and therapeutic index 2.10. The results evidently showed the capacity of Triphala as potential chemopreventive and anticancer drug and immunity.
Highlights
The objective of this study was to compare the stability properties of the two years for the quality of Tri-Pha-La Herbal Drink, Thai traditional and Ayurvedic medicine
Triphala is very important natural ayurvedic formulation, commonly prescribed by most healthcare practitioners in Thailand. It is an anequiproportional mixture of dried fruits of three medicinal herbs, Phyllanthus emblica Linn. (PE), Terminalia bellirica (Gaertn) Roxb. (TB), Terminalia chebula Retz. (TC), known as the ‘Triphala’
The investigation on toxicity which compared fruits of the three plants and Triphala (A.D.17/A.D.18) on P-388, KB, HT 29, MCF-7, A 549, CL and ASK cell showed significantly potential on P-388 (A.D.17); T. chebula-ethyl acetate extract(10.27 g/mL), T. bellirica- ethyl acetate extract (13.12 g/mL), T. bellirica- methanol extract(12.81 g/mL), P. emblica-ethyl acetate extract (14.21 g/mL), P. emblica- methanol extract(14.21 g/mL) and Triphala-methanol extract (13.55 g/mL)
Summary
The objective of this study was to compare the stability properties of the two years for the quality of Tri-Pha-La Herbal Drink, Thai traditional and Ayurvedic medicine. This study aimed to compare the anticancer and anti-HIV efficacy of the solvent extracts and gallic acid of the three fruits. P. emblica-ethyl acetate extract (A.D. 2018) was the most promising anti-syncytium (MC99+1A2) among evaluated all three fruits Triphala extracts: it afforded the lowest EC50 8.85 μM and therapeutic index 2.10. Information on compound and extracts in herbal medicines of triphala formula was acquired from Chae Son National Park, Lampang, a Province of Thailand, in January, 2017 and 2018. With these multiple medicinal properties, especially cytotoxicity and anti-HIVs. The research objectives of this were to comparing those stability properties of the two years for the quality of Tri-Pha-La Herbal Drink. This is a problem solving of poverty and strengthen the economic foundation of farmers to be strong and sustainable economy
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