Abstract
Males of the Drosophila melanogaster mutant croaker (cro) generate a polycyclic pulse song dissimilar to the monocyclic songs typical of wild-type males during courtship. However, cro has not been molecularly mapped to any gene in the genome. We demonstrate that cro is a mutation in the gene encoding the Calmodulin-binding transcription factor (Camta) by genetic complementation tests with chromosomal deficiencies, molecular cloning of genomic fragments that flank the cro-mutagenic P-insertion, and phenotypic rescue of the cro mutant phenotype by Camta+-encoding cDNA as well as a BAC clone containing the gene for Camta. We further show that knockdown of the Camta-encoding gene phenocopies cro mutant songs when targeted to a subset of fruitless-positive neurons that include the mcALa and AL1 clusters in the brain. cro-GAL4 and an anti-Camta antibody labeled a large number of brain neurons including mcALa. We conclude that the Camta-encoding gene represents the cro locus, which has been implicated in a species-specific difference in courtship songs between D. sechellia and simulans.
Highlights
Premating isolation between subpopulations within a species is a hallmark of incipient speciation [1]
In choosing a conspecific male as a mate, fruit fly females rely on a male-derived auditory signal, love song, which is generated by a series of unilateral wing vibration by the male
Our molecular analysis reveals that the cro mutation inhibits expression of the gene encoding a protein called Calmodulin-binding transcription factor (Camta) and that an introduction of the Camta-encoding DNA into the genome of cro mutants allows the mutant male to sing a normal song
Summary
Premating isolation between subpopulations within a species is a hallmark of incipient speciation [1]. A prerequisite to successful premating isolation is the development of a directed bias in mate choice among the members of the species. Choosiness of females has been suggested to operate as one of the major drives for the assortative mating underlying premating isolation, and diversification in male song patterns may result from sexual selection for favorable males [9]. These considerations point to the importance of elucidating the genetic architecture of courtship song patterning mechanisms for the understanding of behavioral evolution
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
