Abstract
We investigated the effects of calcium channel blockers on generation of prostaglandin (PG) E 2 and 6-keto PGF 1α by gastric mucosal surface epithelium. Surface epithelial cells (SEC) isolated from rat gastric mucosa were incubated with either verapamil (1 or 10 μg/ml), diltiazem (2.5 or 25 μg/ml) or nifedipine (2.5 or 25 μg/ml) for 30 min at 37°C in calcium containing or calcium-free medium. Verapamil (both doses)_significantly incerased PGE 2 and 6-keto PGF 1α generation by the surface epithelial cells but only in calcium containing medium. Diltiazem did not affect PG gneration in calcium containing nor calcium-free medium. Nifedipine 25 μg/ml decreased PGE 2 but increased 6-keto PGF 1α generation. The inhibitory effect of nifedipine on GPE 2 generation was abolished in calcium-free medium, while the calmodulin antagonist did not affect verapamil-induced increase in PG generation.
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