Calcium-dependent kinases in the brain have site-specific associations with locomotion and rearing impairments in rats with bile duct ligation

Abstract

We study the impairment of locomotion and rearing behavior in rats with a common bile duct ligation (BDL), and the possible involvement of the PKCγ and CamKIIα gene expression in the brain. Male Wistar rats undergo either sham operation or BDL to induce a rat model of cirrhotic hepatic encephalopathy (HE). Six groups of the animals were divided into three sets of sham-operated and BDL groups. In the first set, locomotion and rearing behavior were assessed on days 1, 7, 14, 21 and 28 of BDL. On day 28 of BDL, blood samples were collected from the second set of the animals for biochemical analysis, and the rats in the third set were used to extract the PFC, the hippocampus, and the cerebellar cortex for examining the Pkcγ and CamKIIα gene expression. The results showed that locomotion and rearing were decreased during 28 days of BDL with the most significant change on the 28th day. Biochemical analysis of the blood revealed hyperammonemia, increases in liver enzymes, and a decrease in albumin indicating liver damage and induction of cirrhotic HE. The results also showed that both of the Pkcγ and CamKIIα gene expressions were increased in the PFC but decreased in the hippocampus. However, the Pkcγ gene expression was decreased but the CamKIIα gene expression was increased in the cerebellar cortex. It can be concluded that the Ca2+-dependent kinases in different brain areas have a site-specific association with the impairment of locomotion and rearing behavior in the cirrhotic HE model rats.