Abstract
Multicellular spheroids are important in biomedical applications, such as drug research and regenerative medicine. We developed microcapsules from sodium alginate and gelatin for mass production of size-controlled spheroids with diameters <200μm. The microcapsules were composed of calcium alginate gels with spherical liquid cores (diameter≈150μm) for formation of spheroids. The spherical liquid cores were prepared by incubating calcium alginate microcapsules containing thermally gelled, cell-enclosing gelatin microparticles about 150μm in diameter, at 37°C. The gelatin microparticles were encapsulated within the microcapsules by dropping a sodium alginate solution containing suspended gelatin microparticles into 100mM CaCl2. The enclosed feline renal fibroblast cell line, CRFK, cells showed 93.8% viability immediately after encapsulation, then grew and completely filled the spherical cores. Multicellular spheroids were collected within 1min by soaking microcapsules in a medium containing alginate lyase.
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