Abstract
Abstract C1q has been purified using precipitation in the presence of chelating agents at low ionic strength. The three times precipitated C1q was highly purified as shown by immunoelectrophoresis and analytic acrylamide disc electrophoresis. On immunoelectrophoresis, the purified C1q showed a single line in the slow γ-region against a potent anti-whole human antiserum, and the hemolytic activity and latex agglutination activity of C1q coincided with this region. The final product showed hemolytic activity and latex agglutination activity but did not show any detectable C1r or C1s activity. The yield of C1q using these methods is about 40 to 60% and thus about 5 mg can be obtained from 100 ml of serum. The highly purified C1q obtained from 3.75% preparative acrylamide gels containing sodium dodecyl sulfate yields potent monospecific antisera when injected into rabbits. These antisera agglutinate EAC1q cells but not EA. The molecular weight of C1q as estimated by its relative mobility on acrylamide gels was 387,600 ± 10,790, and breakdown to smaller subunits has been demonstrated in gels containing urea.
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