Chapter 7 - Broodstock Conditioning and Spawning

Grand monthly means of daily observations of surface sea-water temperature and salinity from twelve light stations along the British Columbia coast during the 13 years 1935 to 1948 have been analysed. In general the temperatures reach a minimum of 45°F. ± 1° (7.2 °C. ± 0.5°) in January and February. The maximum varies from 50° to 64°F. (10° to 18 °C.) in August. The warmest waters occur in bays protected from wind action, and the coldest waters occur in regions of turbulent mixing due to wind or strong currents. The salinity along the mainland coast is a minimum in early summer, associated with the maximum run-off from melting snow. Along the west coast of Vancouver Island the minimum occurs in mid-winter, associated with maximum precipitation which is not stored as snow in this region. At the southern and northern tip of the Queen Charlotte Islands there is little or no variation of salinity because there is no land drainage of consequence in the vicinity.In passes between Georgia Strait and the sea where the waters are mixed to homogeneity by strong tidal currents the annual variation of temperature and salinity is reduced, and in some cases entirely suppressed.On the west coast of Vancouver Island it is shown that the annual cycle is affected by the dominant winds and upwelling of deep ocean waters.

The influence of nutritional and environmental factors on osmoregulation and cataracts in Atlantic salmon ( Salmo salar L)

Unialgal cultures of the macroscopic, vesicular, coenocytic gametophyte (Halicystis parvula Schmitz) of Derbesia tenuissima (DeNotaris) Crouan fr. were grown under various environmental regimes to elucidate the cytology of gamete formation and the factors controlling synchronous gamete formation and release. No synchrony of nuclear division was observed in vegetative plants or during the early stages of gamete formation. In the later stages of gamete formation in plants in a light‐dark cycle, nuclear divisions within any gametangium were synchronous, and the stages of gamete formation were synchronous for the population. This synchrony was not as great for plants in continuous light. Gametes of plants in a light‐dark cycle were released explosively immediately following the dark‐to‐light transition. Release was random and much less forceful for plants in continuous light. After a certain stage of gamete formation, gamete release was timed to occur after a particular interval of darkness, but release could be triggered by light during the last portion of this interval. The length of the dark interval was shorter for male plants than for females, but the period of light sensitivity was longer for females. Formation of gametangia by series of isolated plants was also synchronous and sometimes periodic under certain conditions. Intervals between gametangia on the same plant varied from 2 to 14 days but were usually 4 or 5 days (unlike plants in nature, which show a bi‐ or tri‐weekly periodicity). Male and female plants did not differ in synchrony or periodicity. Different media affected the number of gametangia formed over a period of time but not the synchrony of formation. Under some conditions changing the medium had a stimulating or synchronizing effect. Non‐repeated temperature changes also synchronized gamete formation. Optimum temperature for continued gamete formation was about 21 C. Regular daily light and temperature variation together maintained synchronous and periodic gamete formation in populations of isolated plants. Reproduction diminished and became less synchronous at constant temperature either in continuous light or under a light‐dark schedule, although in the light‐dark regime steps in the formation of any given gametangium remained synchronous with the light‐dark cycle. Length of times between gametangial formation on individual plants showed a tendency to occur in multiples of the usual period lengths; e.g., plants sometimes tend to “skip” intervals, thus maintaining the synchrony of the population. These results suggest that interaction between daily environmental cycles and an endogenous physiological cycle may maintain periodic reproduction.

The hydrology of the coastal waters of Hong Kong

ABSTRACTThe question of what controls gamete release in Ascophyllum nodosum (L.) Le Jolis was studied at six sites along the central coast of Maine. Percent release was assessed weekly along randomly marked transect lines in the mid‐intertidal zone. Six independent variables–water temperature at high tide, air temperature at low tide, nitrogen (ammonia, nitrite, and nitrate), and salinity–were measured concurrently. Stepwise multiple regression analysis on the percentage of plants having released gametes revealed that water temperature at high tide accounted for most of the among‐site variation (R2= 0.77) in the timing of release. The addition of Julian day increased the R2 to 0.82; no other variables were significant. Probit analysis, based on water temperature at high tide, generated an environmentally realistic model for predicting gamete release. The model predicts the onset, midpoint, and termination of gamete release at 6, 10, and 15°C, respectively, and the midpoint at a cumulative water temperature of 358°C. This model has value for developmental studies and, potentially, for reseeding A. nodosum populations. Probits may be useful for characterizing phenological events in other fucoids and algal species.

Annual and spatial variability in gains of body weight in Macoma balthica (L.): Relationships with food supply and water temperature

Brachyuran crabs, like other decapod crustaceans, adopt a number of different strategies for larval dispersal. We verified the influence of variations in temperature, salinity, and pH on the abundance and taxonomic composition of brachyuran larvae in an Amazonian estuary and found evidence of both retention and export dispersal strategies. We identified larvae of 20 different taxa belonging to the families Grapsidae, Ocypodidae, Panopeidae, Pinnotheridae, and Sesarmidae. Ucides cordatus (Linnaeus, 1763) (Ocypodidae), Pachygrapsus gracilis (Saussure, 1857) (Grapsidae), Leptuca cumulanta (Crane, 1943) (Ocypodidae), and Armases rubripes (Rathbun, 1897) (Sesarmidae) were the most abundant species. Most of the taxa present in the study area were at the zoea I stage but later larval stages were found in some species, indicating retention and export. Results were supported by canonical correspondence analysis and general linear model, which related larval community structure and reproduction patterns to variations in salinity, influenced primarily by the enormous discharge of the Amazon River and the high rainfall levels in the region. Further investigations of the distribution of larvae on the continental shelf are necessary to confirm the identified dispersal patterns. The study also presents novel data on the composition, abundance, and dispersal of brachyuran larvae in the tropical estuaries of the Amazon River.

This report provides a synthesis of results obtained between 2005 and 2008 from the Comprehensive Sturgeon Research Program, an interagency collaboration between the U.S. Geological Survey, Nebraska Game and Parks Commission, U.S. Fish and Wildlife Service, and the U.S. Army Corps of Engineers' Missouri River Recovery - Integrated Science Program. The goal of the Comprehensive Sturgeon Research Program is to improve fundamental understanding of reproductive ecology of the pallid sturgeon with the intent that improved understanding will inform river and species management decisions. Specific objectives include: *Determining movement, habitat-use, and reproductive behavior of pallid sturgeon; *Understanding reproductive physiology of pallid sturgeon and relations to environmental conditions; *Determining origin, transport, and fate of drifting pallid sturgeon larvae, and evaluating bottlenecks for recruitment of early life stages; *Quantifying availability and dynamics of aquatic habitats needed by pallid sturgeon for all life stages; and *Managing databases, integrating understanding, and publishing relevant information into the public domain. Management actions to increase reproductive success and survival of pallid sturgeon in the Lower Missouri River have been focused on flow regime, channel morphology, and propagation. Integration of 2005-08 Comprehensive Sturgeon Research Program research provides insight into linkages among flow regime, re-engineered channel morphology, and pallid sturgeon reproduction and survival. The research approach of the Comprehensive Sturgeon Research Program integrates opportunistic field studies, field-based experiments, and controlled laboratory studies. The field study plan is designed to explore the role of flow regime and associated environmental cues using two complementary approaches. An upstream-downstream approach compares sturgeon reproductive behavior between an upstream section of the Lower Missouri River with highly altered flow regime to a downstream section that maintains much of its pre-regulation flow variability. The upstream section also has the potential for an experimental approach to compare reproductive behavior in years with pulsed flow modifications ('spring rises') to years without. The reproductive cycle of the female sturgeon requires several years to progress through gonadal development, oocyte maturation, and spawning. Converging lines of evidence support the hypothesis that maturation and readiness to spawn in female sturgeon is cued many months before spawning. Information on reproductive readiness of shovelnose sturgeon indicates that sturgeon at different locations along the Lower Missouri River between St. Louis and Gavins Point Dam are all responding to the same early cue. Although not a perfect surrogate, the more abundant shovelnose sturgeon is morphologically, physiologically, and genetically similar to pallid sturgeon, and thereby provides a useful comparative model for the rarer species. Day length is the likely candidate to define a temporal spawning window. Within the spawning window, one or more additional, short-term, and specific cues may serve to signal ovulation and release of gametes. Of three potential spawning cues - water temperature, water discharge, and day of year - water temperature is the most likely proximate cue because of the fundamental physiological role temperature plays in sturgeon embryo development and survival, and the sensitivity of many fish hormones to temperature change. It also is possible that neither temperature nor discharge is cueing spawning; instead, reproductive behavior may result from the biological clock advancing an individual fish's readiness to spawn day after day through the spawning period until the right moment, independent of local environmental conditions. Separation of the individual effects of discharge events, water temperature, and other possible factors, such as proximity to male

Rapid thermal adaptation during field temperature variations in Drosophila melanogaster

The Bay Marchand field is located about 60 miles south of New Orleans, Louisiana on the continental shelf. The structurally complex field has produced over 960 MMBOE as of 2012 (http://www.eplweb.com), with many of these hydrocarbons being trapped against the massive salt dome and associated faulting present in the area. A study by Bruno and Hanor (2003) documented the presence of a high salinity plume off the southeast flank of the dome that had less saline and less dense pore water below it. The purpose of this study was to investigate the spatial variations in pore water salinity and temperature on the shallow flanks and crest of the dome that was proposed to be the potential source of the plume mapped in the adjacent Bruno and Hanor (2003) study area. SP and resistivity logs, as well as log header data for 19 boreholes drilled in Bay Marchand were used to calculate salinities, temperatures, and pressures across the area. Results indicate that salt dissolution has led to the presence of four high salinity plumes migrating down-dip away from the dome. Temperature and pressure data suggest the downward migration of seawater as the most likely source of the water supplying the plumes. The plumes appear to be bracketed by major faults in the area. Incorporation of 3D seismic data and fault mapping may be of interest in the future.

Over three million hectares of salt-affected soils characterized with high salinity and sodicity caused serious land degradation in Songnen Plain, northeast China. Soil salinity–sodicity heterogeneous distribution under microtopography is usually influenced by several environmental factors. The side direction movement of soil water driven by water from depression is the key factor that aggravates the soil salinization under microtopography in dry condition. In this study, the differences in surface soil salinity–sodicity (0–10 cm) between dry year and wet year were compared, and the relationship between soil salinity–sodicity and environment factors such as ground elevation, surface ponding time, surface ponding depth, and soil moisture at four soil layers (0–10, 10–30, 30–60, and 60–100 cm) were analyzed using redundancy analysis (RDA) and simple correlation analysis (Pearson analysis) for two different hydrological years. Analyzed soil salinity–sodicity parameters include soluble ions (Na+, K+, Ca2+, Mg2+, CO32−, HCO3−, Cl− and SO42−), salt content (SC), electrical conductivity (EC), sodium adsorption ratio (SAR), and pH. Results showed that values of SAR, Cl−, and SO42− were significantly higher in dry year than in wet year, while Ca2+, Mg2+, K+, and HCO3− showed the opposite results. Values of Na+, CO32−, and EC were significantly higher at higher ground elevation gradient (20–40 cm) in dry year than wet year. Redundancy analysis indicated that spatial distributions and variations of salinity and sodicity in surface soil layer were related with environmental factors of ponding depth, ponding time and ground elevation in wet year, and they were related with ground elevation, ponding depth, ponding time, and soil moisture at 30–60 and 60–100 cm soil layer in dry year. Ponding depth and ground elevation rank first and second as the influential factors of the spatial distribution and variation of soil salinity–sodicity in wet year. However in dry year, primary and secondary influential factors are ground elevation and soil moisture at 60–100 cm soil layer.

Modelling the influence of environmental factors on the physiological status of the Pacific oyster Crassostrea gigas in an estuarine embayment; The Baie des Veys (France)

The brachyuran crabs are iteroparous species which present a high diversification of reproduction patterns, which may have evolved as a species-specific response to environmental conditions. Tropical species commonly present a year-round reproduction due to stable environment conditions. Goniopsis cruentata is a crab species widely distributed along the Western Atlantic, inhabiting practically every microhabitat in the mangrove ecosystem. The aim of the present study is to determine the breeding period of the crab Goniopsis cruentata in Northeastern Brazil and also to evaluate the influence of water salinity, rainfall and air and water temperature on it. A total of 71 ovigerous females, captured from August-2007 to July-2008, were used to assess the breeding period of this species. It was analyzed by the monthly proportion of ovigerous females. A correlation was applied to verify the influence of the abiotic factors on the breeding period. The present population bred seasonal-continuously with peaks in the dry period, which was not associated with monthly variations of salinity, rainfall and air and water temperatures. Therefore, according to statistical analyses, our hypothesis was refuted. However, breeding was intensified in the dry period, when salinity and temperatures were higher and rainfall was lower. We conclude that, even though breeding is not related to monthly variation of environmental factors, it occurs in periods of higher salinity and temperatures and lower rainfall.

This study was conducted at 21 different locations in the Batticaloa lagoon of Sri Lanka to find out the variations of salinity, EC, water temperature and pH at different depth of the lagoon during the month of May 2013 (early dry period). The locations were grouped into four, based on the distance from bar mouth and geographic locations. Vertical profiles of salinity were tested using Salinity Refractrometer. Temperature, pH and Electrical Conductivity (EC) were measured using portable pH/EC/TDS meter. Results were statistically analysed using the Minitab 14 software. Results revealed that, pH varied from 7.87 to 8.89 and 7.98 and 8.79 at the surface and bottom layer of the lagoon, respectively. Result of pH indicated slightly alkaline in nature. Further, observations indicate that the surface layer of lagoon water had higher temperature (mean 32 oC) than the bottom layer (mean 31oC) of lagoon. Furthermore, there was a positive correlation (r=0.146, p=0.168) was found between lagoon depth and salinity.

Summary The reproductive biology of the gregarious brittle star Ophiothrix fragilis is described using the gonad index (GI) and the maturity index (MI) in conjunction with the photoperiod and water temperature. The ophiuroid has a typical annual reproductive cycle going through five distinct reproductive stages. After a resting period during the winter season, the growth and maturation period begins in parallel with a rise in seawater temperature. Spawning takes place during summer after which the gonads regress and the next cycle begins. The rise in temperature seems to influence gonadal growth and more specifically the maturation period of the gametogenic cycle, the indices each attaining higher values earlier in 1999 than in 2000, temperature having reached a maximum a month earlier that year. Representation of the MI in polar coordinates shows a relatively homogenous population during the winter and spring season (resting and maturation periods respectively) while the summer season is more heterogeneous due to the presence of individuals in various reproductive stages. Elevated water temperatures (above 16°C,) mechanical stress believed to be due to strong hydrodynamics and the release of gametes by conspecifics seem to induce spawning in males and females with the former being more sensitive to these cues.