Broiler intestine DMT1 gene expression and bone characteristics, as affected by in ovo injection of different forms of manganese

Abstract

This study was conducted to compare the effects of in ovo injection of different sources of manganese (Mn) on Divalent Metal Transporter 1 (DMT1) gene expression in the small intestine of broilers embryo and hatchling, and its subsequent effects on bone development. A total of 480 fertile eggs (ROSS 308) on embryonic day 17 were divided into 6 treatments with 3 replicates of 20 fertile eggs per replicate. Treatments were included: non-injected (NC) and diluent-injected controls (PC), manganese sulphate (MnS), manganese-methionine (Mn-Met), nano manganese (NMn) and nano manganese-methionine (NMn-Met) groups. Results showed that in ovo injection of different sources of Mn could change the DMT1 mRNA level of intestinal cells (p < .05). DMT1 mRNA level was up-regulated significantly (p < .05) by both nano forms of Mn on embryonic day 18 (E18) and post-hatch day 1 (P1) in all intestinal segments. On post-hatch day 7 (P7), bone length was higher in NMn treatment (p < .05) and tibia ash were affected by NMn and NMn-Met groups (p < .05). On (E18), the percentage of fresh and dry tibia weights were affected by Mn-Met, while on P14, the same characteristics were affected by NMn and MnS treatments, respectively (p < .05). The percentage of Mn in the tibiae ash content significantly (p < .05) enhanced by nano forms of Mn. It is concluded that in ovo injection of nano forms of Mn, either NMn or NMn-Met can be considered as a new strategy to improve Mn absorption in broilers intestine.HighlightsIn ovo injection of nanoparticles led to positive effects on DMT1 mRNA level in different intestinal segment of broilers embryo and hatchling.In ovo injection of nanoparticles enhance the percentage of Mn in the tibiae ash content of broilers embryo and hatchling.In ovo injection of Mn sources improved bone development.