Abstract
Four nonconjugative broad-host-range cloning vectors were derived from the W-plasmid Sa. They are small ( M r 5.6−7.2 × 10 6), carry several drug-resistance markers, and allow constructing and screening for recombinant plasmids generated by the restriction enzymes EcoRI, PstI, BglII, HindIII, BamHI and SalI,
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