Breeding of the new Saccharina variety “Sanhai” with high-yield

Abstract

Backcrossing, self-crossing and target region amplified polymorphism (TRAP) marker assisted selection were utilized to breed a new Saccharina variety in this work. After two continuous cycles of backcrossing using “Fujian” as the recurrent parent and three cycles of self-crossing, the new high-yield variety “Sanhai” with stable excellent traits was obtained. During the whole Saccharina growth period (from January to April), the blade length and width of “Sanhai” were always significantly larger than “Fujian” as the control at the level of 0.05, displaying the obvious growth advantage of the new variety. In addition, the blade width and fresh weigh of “Sanhai” have reached 32.69±3.36cm, 686.82±80.45g respectively which both superior to its two parents (“Fujian” 25.22±4.34cm, 508.51±96.27g and “Rongfu” 24.59±3.58cm, 521.23±68.13g). For this breeding process, TRAP combined with phenotypic traits analysis was used to select the second generation of backcrossing (BC2F1) for subsequent self-crossing. The gene diversities of “Sanhai” obtained in this work (H=0.1572) and “Fujian” (H=0.1687) were lower than that of “Rongfu” (H=0.2303) on the basis of TRAP markers, which showed the outstanding genetic stability of the new variety. The results of Unweighted Pair-Group Method with Arithmetic Mean (UPGMA) cluster indicated variety “Sanhai” could be distinguished from “Fujian” and “Rongfu” and the genetic structure of new variety was stable.