Abstract
Enhancing photosynthetic productivity of rice is always the key goal of researchers by to use exogenous C4 genes. The full-length of intact maize gene of C4-specific PEPCase (ZmC4Ppc) is 6781 bp that makes it difficult for marker-assisted selection (MAS) in rice. The specific marker of intact ZmC4Ppc was designed and termed as MRpc. MRpc was verified that it was a gene marker because ZmC4Ppc could be overexpressed at every developmental stage and it was used for MAS of indica restorer Shuhui881 with ZmC4Ppc and FPM881was gotten, which was tested for genetic background, PEPCase activity, net photosynthetic rate (Pn), general combining ability (GCA) and specific combining ability (SCA). The results indicated that FPM881, which similarity of genetic background had arrived at above 95%, showed a higher PEPCase activity and net photosynthetic rate than those of control. Analysis of yield components showed that some of the progenies carrying ZmC4Ppc had better GCA and SCA than those of the control. Then, six lines of FPM881 were planted in larger population by selfing and gotten selectable-marker free plants with target gene using MRpc and hpt primers. Our results indicated that introduction of ZmC4Ppc via MAS and its stable expression could increase grain yield of hybrid rice.
Highlights
Photosynthesis is main source power of crop production
Shuhui 881 was afforded by Rice Research Institute of Sichuan Agricultural University, Kitaake, a extremely early Japonica variety from Hokkaido of Japan by Mr Fujimoto Kan and Kitaake transformed with ZmC4Ppc by Mr DM Jiao
The genomic DNAs of the materials Yayu 10, Yuyu 22, Linao 4, Kitaake with ZmC4Ppc, Kitaake, Shuhui 881 and FPM 881 were amplified with MRpc by PCR (Fig.2)
Summary
Photosynthesis is main source power of crop production Most of crops, such as rice and wheat, are classified as C3 plants because the first product fixed atmospheric CO2 is 3-PGA. In terrestrial C4 plants, CO2 release from C4 acids and the resulting elevation of cellular CO2 levels take place at a site that is physically separated from the site of initial carboxylation. This separation occurs through the bundle sheath and mesophyll cells in typical/classical C4 plants (Hatch, 1987; Leegood, 2002), and through two distant subcellular compartments in the discovered single-cell C4 plants (Edwards et al, 2004). The key of C4 pathway activation is enzymes relative to CCM
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