Brassicaceous roots as an unexpected diversity hot-spot of helotialean endophytes

Summary1. Coleoptera species show considerable diversity in life histories and ecological strategies, which makes possible their wide distribution in freshwater habitats, including highly stressed ones such as saline or temporary waterbodies. Explaining how particular combinations of traits allow species to occupy distinctive habitats is a central question in ecology.2. A total of 212 sites, sampled over a wide range of inland aquatic habitats in the south‐eastern Iberian Peninsula, yielded 272 species belonging to 68 genera and 11 families. The affinities of genera for 11 biological and 11 ecological traits, gathered from literature and the authors’ own expertise, were used to assess the degree of congruence between taxonomic, biological and ecological traits.3. Taxonomic richness was significantly related to the number of both biological and ecological trait categories, with the richest families also showing the highest functional and ecological diversity. A fuzzy correspondence analysis performed on the abundance‐weighed array of biological traits separated genera according to categories of diet, feeding habits, respiration, reproduction and locomotion. A similar analysis of ecological traits revealed that preferences related to longitudinal distribution (headwater to mouth), local habitat and current velocity best discriminated genera. At the family level, there was a distinctive functional grouping of genera based on biological traits. Only Elmidae showed noticeable homogeneity across genera for both biological and ecological traits.4. Co‐inertia analysis demonstrated a significant match between biological and ecological traits (Rv‐correlation = 0.35, P < 0.001). Elmidae genera displayed the highest concordance, whereas Hydraenidae demonstrated the lowest.5. These results indicate that the predominance of habitat filtering processes in headwater streams yields biological trait conservatism (as shown by Elmidae genera), as well as trait convergence for some specific traits (for instance, respiration) among certain Dytiscidae genera and other typical rheophilic taxa, whereas other biotic factors, such as competition among species, appear more prominent in less stressed habitats. Further knowledge of traits, especially regarding physiological capabilities, is needed to better understand water beetle life history strategies.

Question: What are the differences in trait compositions that enable native plants to colonise comparable natural and man-made habitats? Are these traits independent of phylogenetic relationships between species? Location: Czech Republic. Methods: The relative importance of biological, ecological and distributional traits of native species was studied, using a dataset of 75 species growing in rock and wall habitats in the Czech Republic. Species preferences for individual habitats due to climatic conditions and proportions of different vegetation types in their surroundings were partialled out using partial canonical correspondence analysis. The pattern of plant traits along a gradient from natural rock habitats to secondary wall habitats was analysed using regression trees and generalized linear models with and without phylogenetic correction. Results: The most common native species colonising rock habitats are phanerophytes, mostly woody juveniles, with a CSR life strategy and most are adapted to epizoochory. Summer green leaves, annual life span, CR life strategy, reproduction mostly by seeds and dispersal by ants are all traits positively associated with the ability of species to colonise wall habitats. These species are also characterised by their high demand for nutrients, temperature, base-rich substrates and light. Biological and ecological traits are more important for colonising new habitats than traits related to species dispersal ability or phylogenetic relationships between species. Biological and ecological traits alone explained 29.3% of variability in the species dataset, while dispersal characteristics and phylogeny alone explained 9.1% and 4.8%, respectively. Conclusions: We outline how the process of environmental filtering determines native species assemblages and identify a set of species traits that enable them to persist in particular habitats. We conclude that although urbanisation generally results in loss of natural habitats, there are new, man-made habitats potentially suitable for native species.

Scientific background: Alicyclobacillus spp. includes spore-forming and thermo-acidophilic microorganisms, usually recovered from soil, acidic drinks, orchards and equipment from juice producers. The genus includes different species and sub-species, but A. acidoterrestris is generally regarded as the most important spoiler for acidic drinks and juices. (i) The investigation of microbial community evolution was performed, since soil species could acquire specific phenotypic traits in relation to available nutrients. (ii) A variety of physical and chemical approaches to control A. acidoterrestris were proposed, and the study of sub-lethal injury related to extend of damage was examined. (iii) In vitro stress treatments were tested in vivo. Open questions: (i) Few data are available on the genotyping and phenotyping traits of A. acidoterrestris; (ii) Few data are available on the effect of innovative approaches to control A. acidoterrestris; and no data are available on the sub-lethal injury in A. acidoterrestris after invasive treatments; (iii) Few data are available on the control of A. acidoterrestris in complex conditions. Aims: (i) Selection and characterization of wild strains of A. acidoterrestris as a contribution to species characterization, (ii) studying the structural and functional changes that may affect alicyclobacilli and Bacillus spp. (used as reference) after the application of several sub-lethal physical or chemical treatments, and (iii) validation in a food to assess if an injury could occur also in a complex system. Planning of the research: In the first part, 25 wild strains of A. acidoterrestris from soil (23 strains) and spoiled pear juice (CB-1 and CB-2) were isolated, identified, genotyped and phenotyped. In the second part, 4 strains of A. acidoterrestris (the type strain, 2 wild strains isolated from soil and a wild strain isolated from spoiled pear juice) and 2 species of Bacillus spp. were studied under in vitro conditions after the application of chemical, physical and combined treatments to assess their susceptibility and the presence of sub-lethal injury. The characterization of damage was also performed. Finally, in the last step the presence of sub-lethal injury in vivo was investigated. Materials and Methods: (i) Genotypic traits were performed through examining of 16S rRNA and RAPD PCR (Random-Amplified-Polymophic-DNA). Phenotypical traits were assessed on the opportune laboratory media. (ii) Injury characterization was evaluated by Leakage of UV-absorbing substances and BSA protein assay. (iii) For in vivo assay, clarified apple juice was used. Results: (i) Data of soil-borne strains pinpointed that they could be divided into three blocks, represented by soil strains and by strains moving from soil to other niches. In this context, phenotyping and genotyping did not group the strains in the same way and many strains phylogenetically different showed the same phenotypic trend, thus suggesting that A. acidoterrestris could exist as a genomovar. In addition, the strain CB-1 was distant from other alicyclobacilli, although it possessed the same traits than the other isolate from juice (CB-2); therefore, it is probably a fast-clock organism or the beginning on an alternative pathway in alicyclobacilli evolution. (ii) Alternative approaches applied to control 4 strains of A. acidoterrestris determined a strain-dependent effect probably related to the isolation source; however, the presence of sub-lethal injury related to released nucleic acids, proteins and DPA by spores pointed out the damage on coat and cortex with loss of barrier properties. (iii) The study performed in apple juice confirmed the results obtained in lab media. Significance and Impact of PhD research: From the earliest times, A. acidotererstris was regarded as an important target in the quality control of acidic beverages. Since soil borne species often contaminate fruit juices and do not need strict extreme requirements of acidity and high temperature for survival, it is a great concern to investigate whether and how soil species could evolve from their ecological niches in microbial community to new environments as fruit juices. The present PhD thesis contributed to species characterization through selection and characterization of wild strains of A. acidoterrestris, and provided essential knowledge to validate the genotyping and phenotyping evolution of some strains of A. acidoterrestris. Moreover, this PhD project was the first attempt to investigate the changes that may affect alicyclobacilli after sub-lethal stress application; the release of proteins, nucleic acids and DPA was strictly strain dependent. Finally, the novelty of this PhD thesis was the study of sub-lethal injury on A. acidoterrestris spores in vivo. Future trends: A future perspective could be a focus on a reassessing of strains nomenclature, considering the evolution of some strains of Alicyclobacillus genus, and the different behavior of the type strain compared to the other targets tested.

AimEcological traits (e.g. biotope specialisation and habitat preference) and biological traits (e.g. mobility and life history) are highly variable among species. In insect conservation, considerable focus has been on ecological generalisation, with species occupying many biotope types (generalists) often assumed to be widely distributed, whereas species occupying few biotope types (specialists) are assumed as rare and localised. Although this may be valid in most cases, there is an increasing recognition that functional traits, such as insect mobility and life history, have similar importance as drivers of range size among species. Here, we investigate ecological and biological traits simultaneously to determine their relative importance as drivers of range size variation. We hypothesise that ecological traits are primary drivers, whereas biological traits related to mobility and life history are less important drivers of range size.LocationSub‐Saharan Africa.Taxon115 sub‐Saharan dragonfly species.MethodsFive measures of species range size were obtained from the Odonata Database of Africa. Number of biotope types occupied by adults and larvae, larval habitat preference and adult biological trait information were collated from published sources. We explore the relationships between ecological and biological traits. Using linear mixed models and model averaging, we determine the relative importance of number of biotope types occupied, overall habitat preference and traits related to mobility and life history that drive dragonfly range size while accounting for phylogenetic relationships among focal species.ResultsDragonflies occupying more biotope types had wider ranges, although these relationships were overall weak. Dragonflies with longer and later breeding seasons had wider ranges. Overall habitat preference and mobility traits had low importance in our models.Main conclusionsEcological versus biological traits are interactive in shaping the geographical ranges of dragonfly species across Africa. Single traits are weak predictors of species range sizes and we recommend investigating multiple traits simultaneously to improve the accuracy of predictions. In doing so, more informed decisions can be made to ensure effective large‐scale conservation of dragonflies.

Genome size (C-value) and endopolyploidy (endoreduplication index, EI) are known to correlate with various morphological and ecological traits, in addition to phylogenetic placement. A phylogenetically controlled multivariate analysis was used to explore the relationships between DNA content and phenotype in angiosperms. Seeds from 41 angiosperm species (17 families) were grown in a common glasshouse experiment. Genome size (2C-value and 1Cx-value) and EI (in four tissues: leaf, stem, root, petal) were determined using flow cytometry. The phylogenetic signal was calculated for each measure of DNA content, and phylogenetic canonical correlation analysis (PCCA) explored how the variation in genome size and EI was correlated with 18 morphological and ecological traits. Phylogenetic signal (λ) was strongest for EI in all tissues, and λ was stronger for the 2C-value than the 1Cx-value. PCCA revealed that EI was correlated with pollen length, stem height, seed mass, dispersal mechanism, arbuscular mycorrhizal association, life history and flowering time, and EI and genome size were both correlated with stem height and life history. PCCA provided an effective way to explore multiple factors of DNA content variation and phenotypic traits in a phylogenetic context. Traits that were correlated significantly with DNA content were linked to plant competitive ability.

Golden camellia species are endangered species with great ecological significance and economic value in the section Chrysantha of the genus Camellia of the family Theaceae. Literature shows that more than 50 species of golden camellia have been found all over the world, but the exact number remains undetermined due to the complex phylogenetic background, the non-uniform classification criteria, and the presence of various synonyms and homonyms; and phylogenetic relationships among golden camellia species at the gene level are yet to be disclosed. Therefore, it is necessary to investigate the divergence time and phylogenetic relationships between all golden camellia species at the gene level to improve their classification system and achieve accurate identification of them. Phenotypic data and transcriptomic sequences of 20 golden camellia species commonly found in Guangxi, China were obtained. PCA and OPLS-DA analyses were conducted based on phenotypic data, and agglomerative clustering was performed to generate the clustering tree of the 20 golden camellia species. Single-copy homologous genes were used to generate phylogenetic trees using Neighbor-Joining, Maximum Likelihood, and Bayesian Inference methods, and the results obtained with these three methods were compared. Then the molecular dating analysis was performed to reveal the divergence time and evolutionary relationships. Rhododendron griersonianum, Diospyros lotus, and Impatiens glandulifera were used as outgroups. The phylogenetic tree based on single-copy homologous genes showed that golden camellia species with shorter geographical distances were closer phylogenetically. Phylogenetic relationships based on phenotypic traits and those based on single-copy homologous genes were inconsistent, suggesting that species with a close genetic evolutionary relationship may show high variation in phenotypic traits and thus the analysis of evolutionary relationships based on phenotypic traits may result in inaccurate outcomes. Among three phylogenetic trees constructed by the three methods, the evolutionary sequences were different, but evolutionary relationships between most species were consistent. For 6 species, the divergence time estimated by Maximum Likelihood and Bayesian Inference varied much, that estimated by Bayesian Inference later than that estimated by Maximum Likelihood. Using these two methods, the resulting divergence time of 14 species was 3.452 Mya. The divergence time predicted in our study is later than that in the literature. In the present study phylogenetic relationships among 20 golden camellia species were analyzed at the transcriptome level to provide a supplement to the phylogenetic classification and evolutionary relationships explored using morphological traits and some molecular markers. Our findings show that the 20 golden camellia species diverged at a later time than other known species in the genus Camellia. Since our analyses were based on the failed molecular clock hypothesis, our conclusions are tentative. Further research using more systematic analyses and more methods should be conducted to confirm the phylogenetic relationships among golden camellia species.

1 Relating species traits to habitat characteristics can provide important insights into the structure and functioning of stream communities. However, trade-offs among species traits make it difficult to predict accurately the functional diversity of freshwater communities. Many authors have pointed to the value of working with groups of organisms as similar as possible in terms of relationships among traits and have called for definition of groups of organisms with similar suites of attributes. 2 We used multivariate analyses to examine separately the relationships among 11 biological traits and among 11 ecological traits of 472 benthic macroinvertebrate taxa (mainly genera). The main objective was to demonstrate (1) potential trade-offs among traits; (2) the importance of the different traits to separate systematic units or functional groupings; and (3) uniform functional groups of taxa that should allow a more effective use of macroinvertebrate biological and ecological traits. 3 We defined eight groups and 15 subgroups according to a biological trait ordination which highlighted size (large to small), reproductive traits (K to r strategists), food (animal to plant material) and feeding habits (predator to scraper and/or deposit feeder) as ‘significant’ factors determining the ordination of taxa. This ordination partly preserved phylogenetic relationships among groups. 4 Seven ecological groups and 13 ecological subgroups included organisms with combinations of traits which should be successively more adequate in habitats from the main channel to temporary waters, and from the crenon to the potamic sections of rivers, and to systems situated outside the river floodplain. These gradients corresponded to a gradual shift from (1) rheophilic organisms that lived in the main channel of cold oligotrophic mountain streams to (2) animals that preferred eutrophic habitats of still or temporary waters in lowlands. The groups with similar ecological traits had a more diverse systematic structure than those with similar biological traits. 5 Monitoring and assessment tools for the management of water resources are generally more effective if they are based on a clear understanding of the mechanisms that lead to the presence or absence of species groups in the environment. We believe that groups with similar relationships among their species traits may be useful in developing tools that measure the functional diversity of communities.

We use cross-national, longitudinal data to explore the impact of educational level on changes in health outcomes among Europeans aged over 50. Our analyses are performed separately for Northern, Western and Southern Europe, as these regions broadly represent different welfare state regimes. We find that low education is associated with higher incident events — over a two-year period — of poor health, chronic diseases and disability, but it is less consistently associated with new events of long-standing illness. Net of behavioural risk factors, educational effects are more consistent in Western and Southern Europe than in the Nordic welfare states. In Northern Europe, lower education is associated with less financial and employment disadvantage than in Southern or Western Europe. After controlling for educational differences in these factors, effects of educational level on health deterioration remain significant for most outcomes in Western and Southern Europe, whereas they are weaker and non-significant after adjustment in Northern Europe.

Summary A phenotypic syndrome refers to complex patterns of integration among functionally related traits in an organism that defines how the organism interacts with its environment and sustains itself. Human‐induced biological invasions have become important sources of environmental modifications. However, the extent to which invasive species affect the phenotypic syndromes of individuals in a native is currently unknown. Such knowledge has important implications for understanding ecological interactions and the management of biological invasions. Here, field monitoring in a natural stream were combined with standardized estimates of behavioral, physiological and morphological traits to address the hypothesis that coexistence with a non‐native invader induces a novel environmental pressure that disrupts the adaptive integration among phenotypic traits of the native species. We compared the strength of integration among key phenotypic traits (i.e. aerobic scope, standard metabolic rate, body growth, activity, and body shape) and ecological niche traits (i.e. spring and summer diet, home range size, daily movements) of an allopatric group of native brown trout (Salmo trutta) with a group of brown trout living in sympatry with non‐native brook trout (Salvelinus fontinalis). We found that the integration of phenotypic traits was substantially reduced in the sympatric brown trout and that allopatric and sympatric brown trout differed in key phenotypic and ecological niche traits. Brown trout living in sympatry with non‐native brook trout consumed more terrestrial prey, had smaller home ranges, and a stouter body shape. Sympatric brown trout also had lower specific growth rate, suggesting a lower fitness. The results are generally in line with our hypothesis suggesting that the reduction in fitness observed in sympatric brown trout is caused by the breakdown of their adaptive phenotypic syndrome. This may be caused by differences in the plasticity of the response of phenotypic traits to the novel selection pressure induced by the non‐native species. Our results may help explaining deleterious effects of non‐native species reported in the absence of direct competition with the native species. A lay summary is available for this article.

A cryptic radiation of Caribbean sea slugs revealed by integrative analysis: Cyerce 'antillensis' (Sacoglossa: Caliphyllidae) is six distinct species.

The phylogenetic variations of fine root traits, which are related to plant growth and development as well as to physiological and ecological processes, are not fully understood. This study aimed to: (1) examine how tree species and sampling methodology affect the anatomical, morphological and nutrient traits of fine roots; and (2) determine whether phylogenetic signals affect fine root trait relationships and influence comparison of root traits between the branch order-based and diameter-based cut-off sampling categories. Fine root samples of 16 subtropical forest tree species were obtained and their anatomical, morphological and nutrient traits were studied. The phylogenetic signals of trait variations were calculated to determine trait relationships. Tree species and sampling methodology significantly affected fine root traits (p < 0.05). Mean root diameters, root tissue density (RTD) and carbon-to-nitrogen ratio were the lowest in the first-order category and highest in the ≤2 mm category. The reverse pattern was found for specific root length, specific root area and nitrogen concentration. Morphological traits showed significant phylogenetic signals; however, nutrient traits did not reflect phylogenetic conservatism. Phylogenetic factors influenced correlations between traits for the first-order root economics spectrum. Root traits were multidimensional and RTD was loaded on a novel phylogenetic principal component analysis. Functional traits of fine roots are multidimensional for subtropical tree species and closely linked to phylogeny. Morphological traits of first order roots showed a much stronger phylogenetic signal than those of roots ≤2 mm (traditionally defined fine roots). The findings improve understanding of root trait strategies in response to environmental changes.

BackgroundBacillus cereus sensu lato (s. l.) is an ecologically diverse bacterial group of medical and agricultural significance. In this study, I use publicly available genomes and novel bioinformatic workflows to characterize the B. cereus s. l. pan-genome and perform the largest phylogenetic and population genetic analyses of this group to date in terms of the number of genes and taxa included. With these fundamental data in hand, I identify genes associated with particular phenotypic traits (i.e., “pan-GWAS” analysis), and quantify the degree to which taxa sharing common attributes are phylogenetically clustered.MethodsA rapid k-mer based approach (Mash) was used to create reduced representations of selected Bacillus genomes, and a fast distance-based phylogenetic analysis of this data (FastME) was performed to determine which species should be included in B. cereus s. l. The complete genomes of eight B. cereus s. l. species were annotated de novo with Prokka, and these annotations were used by Roary to produce the B. cereus s. l. pan-genome. Scoary was used to associate gene presence and absence patterns with various phenotypes. The orthologous protein sequence clusters produced by Roary were filtered and used to build HaMStR databases of gene models that were used in turn to construct phylogenetic data matrices. Phylogenetic analyses used RAxML, DendroPy, ClonalFrameML, PAUP*, and SplitsTree. Bayesian model-based population genetic analysis assigned taxa to clusters using hierBAPS. The genealogical sorting index was used to quantify the phylogenetic clustering of taxa sharing common attributes.ResultsThe B. cereus s. l. pan-genome currently consists of ≈60,000 genes, ≈600 of which are “core” (common to at least 99% of taxa sampled). Pan-GWAS analysis revealed genes associated with phenotypes such as isolation source, oxygen requirement, and ability to cause diseases such as anthrax or food poisoning. Extensive phylogenetic analyses using an unprecedented amount of data produced phylogenies that were largely concordant with each other and with previous studies. Phylogenetic support as measured by bootstrap probabilities increased markedly when all suitable pan-genome data was included in phylogenetic analyses, as opposed to when only core genes were used. Bayesian population genetic analysis recommended subdividing the three major clades of B. cereus s. l. into nine clusters. Taxa sharing common traits and species designations exhibited varying degrees of phylogenetic clustering.ConclusionsAll phylogenetic analyses recapitulated two previously used classification systems, and taxa were consistently assigned to the same major clade and group. By including accessory genes from the pan-genome in the phylogenetic analyses, I produced an exceptionally well-supported phylogeny of 114 complete B. cereus s. l. genomes. The best-performing methods were used to produce a phylogeny of all 498 publicly available B. cereus s. l. genomes, which was in turn used to compare three different classification systems and to test the monophyly status of various B. cereus s. l. species. The majority of the methodology used in this study is generic and could be leveraged to produce pan-genome estimates and similarly robust phylogenetic hypotheses for other bacterial groups.

Biodiversity is a multifaceted concept for which the functional component, that influences many aspects of ecosystem functioning, remains difficult to characterise. In several taxa, many studies rely on morphological traits as proxies of species ecological attributes. However, the extent to which morphology provides a good surrogate of ecological traits has rarely been acknowledged.Here, we used morphological and ecological trait databases to test whether morphological traits are linked to ecological traits and can be used to assess various biodiversity facets in 254 stream fish communities across France.We firstly computed co‐inertia analyses to test the association between morphological and ecological traits related to either habitat use, feeding or life‐history strategies. We then computed a suite of diversity indices to investigate the relationship between morphological and ecological indices.We found a strong association between morphological and ecological traits, and significant relationships between diversity indices computed on morphological and ecological traits. However, these relationships varied according to the index and type of ecological traits, with the strongest relationship observed with traits related to habitat use.Although these results highlight that the use of morphological data is a promising way to understand ecological diversity, they also reveal that the choice of diversity index and the type of ecological traits targeted are particularly important and need to be taken into consideration.

Increasing corn grain production without devastating new forest areas is a viable alternative to controlling deforestation. However, increasing plant density in the area may alter plant morphophysiological and productive traits. The objective of this study was to characterize relationships between physiological, morphological and yield traits of corn plants, as well as the cause, effect and relationship of the traits on grain yield. The experiment was carried out in randomized complete block design with four replications. The corn hybrids (2B655PW, AG7088PRO3 and P4285YHR) were grown with row spacing of 40 cm, 60 cm, 80 cm, 95 cm. The evaluated traits physiological were: net photosynthesis (PN), stomatal conductance (Gs), intercellular concentration of CO2 (Ci), leaf transpiration (E), water use efficiency (WUE) and carboxylation efficiency (CE). The morphological were: plant height (PH) and ear insertion height (EIH), stem diameter (SD), and leaf area (LA) and the productive traits were the total number of ear per hectare (NE), number of grains per ear (NGE), grain mass per ear (GME), 100 grain weight (100GW) and grain yield (GY). The characteristics of maize hybrids cultivated in environment with reduced spacing (40cm, 60cm, 80cm, 95cm) of the 2016/2017 crop were investigated through the multicollinearity path analysis. The physiological, morphological and productive traits are considered sources of variation of cause and effect of corn yield in reduced spaced. This traits are essential for observations in maize breeding programs to obtain high yielding varieties in reduced spacing. In conclusion, the physiological (PN, CE, WUE, Ci, Gs), morphological (EIH, SD, LA) and productive (NE, GME) traits provide gains in maize grain yield via indirect selection when the crop is subjected to 40 cm row spacing.

1. Current budgets for environmental management are high, tend to increase, and are used to support policy and legislation which is standardized for large geographic units. Therefore, the search for tools to monitor the effects of this investment is a major issue in applied ecology. Ideally, such a biomonitoring tool should: (1) be as general as possible with respect to its geographic application; (2) be as specific as possible by separating different types of human impact on a given ecosystem; (3) reliably indicate changes in human impact of a particular type; and (4) be derived from a sound theoretical concept in ecology.2. We developed an approach to biomonitoring which matches these ‘ideal’ characteristics by focusing on numerous, general biological species traits (e.g. size, number of descendants per reproductive cycle, parental care, mobility) and on the habitat templet concept, which relates trends in these general species traits to disturbance patterns. Using the French Rhône River and benthic macroinvertebrates as an example, we have used the data to demonstrate a general framework and the potential of our approach rather than to produce a ready‐made tool. Our data covered a large river and its major tributaries, which has a catchment that crosses ecoregions, and known gradients and discontinuities in human impact.3. We applied multivariate analyses to evaluate how the distribution of species traits in invertebrate communities could discriminate environmental differences along the Rhône in comparison to traditionally used approaches (e.g. community structure, based on species abundances, or ecological species traits, such as velocity preferences and pollution tolerance). Invertebrate community structure expressed in terms either of the abundance or the traits of species reliably indicated differences in overall human impact. The community structure based on biological traits was less confounded by natural spatial gradients and reliably indicated human impact, while community structure based on ecological traits was the most confounded by natural spatial gradients and was the poorest indicator of human impact. Community structure based on species abundances was an intermediate indicator of human impact.4. These results indicate that a revision of biomonitoring approaches which have been based on a single aspect of the biological responses may be warranted. The biological traits of species could separate the different types of human impact. Therefore, the use of these traits in biomonitoring could improve existing multi‐metric approaches. Future research has to show if the general applicability of species traits allows the development of a unique biomonitoring tool for running waters of the European Union, for running waters in temperate climates on several continents, for freshwater, marine and terrestrial systems, and/or for global biodiversity assessment.