Abstract
Aim:The study aimed to assess the nature of animal origin Staphylococcus aureus strains. The study has zoonotic importance and aimed to compare virulence between two different hosts, i.e., bovine and ovine origin.Materials and Methods:Conventional polymerase chain reaction-based methods used for the characterization of S. aureus strains and chick embryo model employed for the assessment of virulence capacity of strains. All statistical tests carried on R program, version 3.0.4.Results:After initial screening and molecular characterization of the prevalence of S. aureus found to be 42.62% in bovine origin samples and 28.35% among ovine origin samples. Meanwhile, the methicillin-resistant S. aureus prevalence is found to be meager in both the hosts. Among the samples, only 6.8% isolates tested positive for methicillin resistance. The biofilm formation quantified and the variation compared among the host. A Welch two-sample t-test found to be statistically significant, t=2.3179, df=28.103, and p=0.02795. Chicken embryo model found effective to test the pathogenicity of the strains.Conclusion:The study helped to conclude healthy bovines can act as S. aureus reservoirs. Bovine origin S. aureus strains are more virulent than ovine origin strains. Bovine origin strains have high probability to become zoonotic pathogen. Further, gene knock out studies may be conducted to conclude zoonocity of the bovine origin strains.
Highlights
Staphylococcus aureus is a major opportunistic pathogen responsible for wide range of infections
The nasal swabs collected were initially spread on Mannitol salt agar (MSA) and Baird-Parker Agar, a selective media for S. aureus
The colonies with yellow to golden yellow color on MSA plates and black colonies on Baird-Parker agar plates selected for further screening. 30 samples from the bovine origin and 20 samples from ovine origin were positive on both selective media agar plates
Summary
Staphylococcus aureus is a major opportunistic pathogen responsible for wide range of infections. The ability of S. aureus to cause diseases in humans and animals during immune compromised times has been attributed to its capacity to produce a variety of virulence factors [1]. The epidemiological circumstances changed mainly with the advent and spread of community-acquired methicillin-resistant S. aureus (CA-MRSA) strains, appearing in individuals without healthcare-associated risk factors and in animals. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
