Abstract
The aim of this report was to investigate the behavior of human mesenchymal stem cells (hMSCs) when cultured on different porous 3D scaffolds, having natural or synthetic origination. Natural scaffolds were obtained by hand made processing of human bone tissue (allograft), because its well known osteoconductive features, using different procedures to eliminate the donor cellular phase. Cancellous bone was frozen, heated or demineralized before being loaded with hMSCs. Among the variety of synthetic materials, biodegradable polymeric spongy matrices were chosen and comparatively tested as scaffolds for hMSCs growth.
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